PMID- 10068651
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20131121
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 93
IP  - 6
DP  - 1999 Mar 15
TI  - Gab-family adapter proteins act downstream of cytokine and growth factor
      receptors and T- and B-cell antigen receptors.
PG  - 1809-16
AB  - We previously found that the adapter protein Gab1 (110 kD) is
      tyrosine-phosphorylated and forms a complex with SHP-2 and PI-3 kinase upon
      stimulation through either the interleukin-3 receptor (IL-3R) or gp130, the
      common receptor subunit of IL-6-family cytokines. In this report, we identified
      another adapter molecule (100 kD) interacting with SHP-2 and PI-3 kinase in
      response to various stimuli. The molecule displays striking homology to Gab1 at
      the amino acid level; thus, we named it Gab2. It contains a PH domain,
      proline-rich sequences, and tyrosine residues that bind to SH2 domains when they 
      are phosphorylated. Gab1 is phosphorylated on tyrosine upon stimulation through
      the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell 
      and B-cell antigen receptors (TCR and BCR, respectively), in addition to IL-3R
      and gp130. Tyrosine phosphorylation of Gab2 was induced by stimulation through
      gp130, IL-2R, IL-3R, TPOR, SCFR, and TCR. Gab1 and Gab2 were shown to be
      substrates for SHP-2 in vitro. Overexpression of Gab2 enhanced the gp130 or
      Src-related kinases-mediated ERK2 activation as that of Gab1 did. These data
      indicate that Gab-family molecules act as adapters for transmitting various
      signals.
FAU - Nishida, K
AU  - Nishida K
AD  - Division of Molecular Oncology, Biomedical Research Center, Osaka University
      Medical School, Osaka, Japan.
FAU - Yoshida, Y
AU  - Yoshida Y
FAU - Itoh, M
AU  - Itoh M
FAU - Fukada, T
AU  - Fukada T
FAU - Ohtani, T
AU  - Ohtani T
FAU - Shirogane, T
AU  - Shirogane T
FAU - Atsumi, T
AU  - Atsumi T
FAU - Takahashi-Tezuka, M
AU  - Takahashi-Tezuka M
FAU - Ishihara, K
AU  - Ishihara K
FAU - Hibi, M
AU  - Hibi M
FAU - Hirano, T
AU  - Hirano T
LA  - eng
SI  - GENBANK/AB018413
SI  - GENBANK/AB018414
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, CD)
RN  - 0 (GAB2 protein, human)
RN  - 0 (Gab1 protein, mouse)
RN  - 0 (Gab2 protein, mouse)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Il6st protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Interleukin-3)
RN  - 0 (Receptors, Thrombopoietin)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - 143641-95-6 (MPL protein, human)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 3.1.3.48 (PTPN11 protein, human)
RN  - EC 3.1.3.48 (PTPN6 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpn11 protein, mouse)
RN  - EC 3.1.3.48 (Ptpn6 protein, mouse)
SB  - AIM
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/physiology
MH  - Cell Line
MH  - Cytokine Receptor gp130
MH  - Gene Expression
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Glycoproteins/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoproteins/chemistry/genetics/*metabolism
MH  - Phosphorylation
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/metabolism
MH  - Proto-Oncogene Proteins/physiology
MH  - Proto-Oncogene Proteins c-kit/physiology
MH  - Receptors, Antigen, B-Cell/physiology
MH  - Receptors, Antigen, T-Cell/physiology
MH  - *Receptors, Cytokine
MH  - Receptors, Interleukin-3/physiology
MH  - Receptors, Thrombopoietin
MH  - Signal Transduction
MH  - Tyrosine/metabolism
EDAT- 1999/03/09 00:00
MHDA- 1999/03/09 00:01
CRDT- 1999/03/09 00:00
PHST- 1999/03/09 00:00 [pubmed]
PHST- 1999/03/09 00:01 [medline]
PHST- 1999/03/09 00:00 [entrez]
PST - ppublish
SO  - Blood. 1999 Mar 15;93(6):1809-16.