PMID- 10068040
OWN - NLM
STAT- MEDLINE
DCOM- 19990315
LR  - 20190826
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 35
IP  - 16
DP  - 1998 Nov
TI  - The mouse genome contains two expressed intronless retroposed pseudogenes for the
      sentrin/sumo-1/PIC1 conjugating enzyme Ubc9.
PG  - 1057-67
AB  - The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like
      peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We 
      show that the mouse genome contains four copies of the ubc-9 gene. These include 
      a structural ubc-9 gene consisting of seven exons which encode a protein
      identical to human ubc-9, and three intronless processed pseudogenes. The open
      reading frames (ORF) of two of the pseudogenes, ubc9-psi1 and ubc9-psi2,
      correspond to the cDNA of ubc-9 and encode for proteins which differ from ubc9 by
      three and one amino acid substitutions respectively. The third pseudogene,
      ubc9-psi3, contains many mutations and stop codons. ubc9-psi1 and ubc9-psi2 are
      flanked by 5'- and 3'-untranslated (UT) regions homologous to those of the
      structural ubc-9 gene. Both genes contain a polyA tail and direct repeats at both
      ends suggesting that they arose by mRNA retroposition. Both ubc9-psi1 and
      ubc9-psi2 are transcribed into mRNA in murine cells. In contrast to ubc9, the
      protein products of ubc9-psil and ubc9-psi2 fail to bind Fas and to complement an
      yeast conditional ubc9 mutant. These results suggest that ubc9-psi1 and ubc9-psi2
      encode for proteins that may interact with targets that differ from those
      recognized by ubc-9.
FAU - Tsytsykova, A V
AU  - Tsytsykova AV
AD  - Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston,
      MA 02115, USA.
FAU - Tsitsikov, E N
AU  - Tsitsikov EN
FAU - Wright, D A
AU  - Wright DA
FAU - Futcher, B
AU  - Futcher B
FAU - Geha, R S
AU  - Geha RS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Retroelements)
RN  - 0 (SUMO-1 Protein)
RN  - 0 (Ubiquitins)
RN  - 0 (fas Receptor)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (ubiquitin-conjugating enzyme UBC9)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Exons
MH  - Gene Expression
MH  - Genes
MH  - Genetic Complementation Test
MH  - Genome
MH  - Humans
MH  - Introns
MH  - Ligases/*genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Pseudogenes
MH  - RNA, Messenger/genetics/metabolism
MH  - Restriction Mapping
MH  - Retroelements
MH  - SUMO-1 Protein
MH  - Saccharomyces cerevisiae/genetics
MH  - *Ubiquitin-Conjugating Enzymes
MH  - Ubiquitins/metabolism
MH  - fas Receptor/metabolism
EDAT- 1999/03/06 03:12
MHDA- 2000/05/20 09:00
CRDT- 1999/03/06 03:12
PHST- 1999/03/06 03:12 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 1999/03/06 03:12 [entrez]
AID - S0161589098000947 [pii]
AID - 10.1016/s0161-5890(98)00094-7 [doi]
PST - ppublish
SO  - Mol Immunol. 1998 Nov;35(16):1057-67. doi: 10.1016/s0161-5890(98)00094-7.