PMID- 10066797
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 11
DP  - 1999 Mar 12
TI  - Cell cycle-dependent expression and centrosome localization of a third human
      aurora/Ipl1-related protein kinase, AIK3.
PG  - 7334-40
AB  - We earlier isolated cDNAs encoding novel human protein kinases AIK and AIK2
      sharing high amino acid sequence identities with Drosophila Aurora and
      Saccharomyces cerevisiae Ipl1 kinases whose mutations cause abnormal chromosome
      segregation. In the present study, a third human cDNA (AIK3) highly homologous to
      aurora/IPL1 was isolated, and the nucleotide sequence was determined. This cDNA
      encodes 309 amino acids with a predicted molecular mass of 35.9 kDa. C-terminal
      kinase domain of AIK3 protein shares high amino acid sequence identities with
      those of Aurora/Ipl1 family protein kinases including human AIK, human AIK2,
      Xenopus pEg2, Drosophila Aurora, and yeast Ipl1, whereas the N-terminal domain of
      AIK3 protein shares little homology with any other Aurora/Ipl1 family members.
      AIK3 gene was assigned to human chromosome 19q13.43, which is a frequently
      deleted or rearranged region in several tumor tissues, by fluorescence in situ
      hybridization, somatic cell hybrid panel, and radiation hybrid cell panel.
      Northern blot analyses revealed that AIK3 expression was limited to testis. The
      expression levels of AIK3 in several cancer cell lines were elevated severalfold 
      compared with normal fibroblasts. In HeLa cells, the endogenous AIK3 protein
      level is low in G1/S, accumulates during G2/M, and reduces after mitosis.
      Immunofluorescence studies using a specific antibody have shown that AIK3 is
      localized to centrosome during mitosis from anaphase to cytokinesis. These
      results suggest that AIK3 may play a role(s) in centrosome function at later
      stages of mitosis.
FAU - Kimura, M
AU  - Kimura M
AD  - Department of Molecular Pathobiochemistry, Gifu University School of Medicine,
      Tsukasamachi-40, Gifu 500-8705, Japan.
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Yoshioka, T
AU  - Yoshioka T
FAU - Okano, Y
AU  - Okano Y
LA  - eng
SI  - GENBANK/AB017332
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.11.1 (AURKB protein, human)
RN  - EC 2.7.11.1 (Aurora Kinase B)
RN  - EC 2.7.11.1 (Aurora Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Aurora Kinase B
MH  - Aurora Kinases
MH  - Base Sequence
MH  - Blotting, Western
MH  - *Cell Cycle
MH  - Centrosome/*enzymology
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 19
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Protein-Serine-Threonine Kinases/*genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/enzymology
MH  - Testis/enzymology
MH  - Tumor Cells, Cultured
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - 10.1074/jbc.274.11.7334 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 12;274(11):7334-40. doi: 10.1074/jbc.274.11.7334.