PMID- 10066796
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 11
DP  - 1999 Mar 12
TI  - An ankyrin-like protein with transmembrane domains is specifically lost after
      oncogenic transformation of human fibroblasts.
PG  - 7325-33
AB  - We have identified a novel transformation-sensitive mRNA, which is present in
      cultured fibroblasts but is lacking in SV40 transformed cells as well as in many 
      mesenchymal tumor cell lines. The corresponding gene is located on human
      chromosome 8 in band 8q13. The open reading frame of the mRNA encodes a protein
      of 1119 amino acids forming two distinct domains. The N-terminal domain consists 
      of 18 repeats that are related to the cytoskeletal protein ankyrin. The
      C-terminal domain contains six putative transmembrane segments that resemble many
      ion channels. This overall structure is reminiscent of TRP-like proteins that
      function as store-operated calcium channels. The novel protein with an Mr of 130 
      kDa is expressed at a very low level in human fibroblasts and at a moderate level
      in liposarcoma cells. Overexpression in eukaryotic cells appears to interfere
      with normal growth, suggesting that it might play a direct or indirect role in
      signal transduction and growth control.
FAU - Jaquemar, D
AU  - Jaquemar D
AD  - M. E. Muller Institute, University of Bern, CH-3010 Bern, Switzerland.
FAU - Schenker, T
AU  - Schenker T
FAU - Trueb, B
AU  - Trueb B
LA  - eng
SI  - GENBANK/Y10601
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Base Sequence
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Chromosomes, Human, Pair 8
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Fibroblasts/cytology/metabolism
MH  - Humans
MH  - Ion Channels/genetics/metabolism
MH  - Membrane Proteins/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - *Oncogenes
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - 10.1074/jbc.274.11.7325 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 12;274(11):7325-33. doi: 10.1074/jbc.274.11.7325.