PMID- 10066450
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20051117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 256
IP  - 1
DP  - 1999 Mar 5
TI  - Three splicing variants of tomosyn and identification of their syntaxin-binding
      region.
PG  - 218-22
AB  - We have recently isolated a neural tissue-specific syntaxin-1-binding protein,
      named tomosyn, which is capable of dissociating Munc18/n-Sec1/rbSec1 from
      syntaxin-1 to form a 10S tomosyn complex, an intermediate complex converted to
      the 7S SNARE complex. We isolated here two splicing variants of tomosyn: one had 
      36 amino acids (aa) insertion and another had 17 aa deletion. We named original
      one m-tomosyn, big one b-tomosyn, and small one s-tomosyn. s-Tomosyn as well as
      m-tomosyn was mainly expressed in brain whereas b-tomosyn was ubiquitously
      expressed. All the isoforms bound to syntaxin-1, but not to syntaxin-2, -3, or
      -4, and had a region highly homologous to VAMP, another syntaxin-binding protein.
      This region was necessary but not sufficient for high-affinity binding of tomosyn
      to syntaxin-1.
CI  - Copyright 1999 Academic Press.
FAU - Yokoyama, S
AU  - Yokoyama S
AD  - Department of Molecular Biology and Biochemistry, Osaka University Medical
      School, Suita, 565-0871, Japan.
FAU - Shirataki, H
AU  - Shirataki H
FAU - Sakisaka, T
AU  - Sakisaka T
FAU - Takai, Y
AU  - Takai Y
LA  - eng
SI  - GENBANK/AF118889
SI  - GENBANK/AF118890
SI  - GENBANK/U92072
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, Surface)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Isoforms)
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (R-SNARE Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Stx1a protein, rat)
RN  - 0 (Stxbp5 protein, rat)
RN  - 0 (Syntaxin 1)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cloning, Molecular
MH  - *Gene Expression
MH  - Gene Library
MH  - Kidney
MH  - Membrane Proteins/chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/metabolism
MH  - Neuropeptides/chemistry/*genetics/metabolism
MH  - Organ Specificity
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Binding
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - Qa-SNARE Proteins
MH  - R-SNARE Proteins
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Syntaxin 1
MH  - *Vesicular Transport Proteins
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - S0006-291X(99)90300-5 [pii]
AID - 10.1006/bbrc.1999.0300 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Mar 5;256(1):218-22. doi:
      10.1006/bbrc.1999.0300.