PMID- 10066432
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 256
IP  - 1
DP  - 1999 Mar 5
TI  - Shedding of CD163, a novel regulatory mechanism for a member of the scavenger
      receptor cysteine-rich family.
PG  - 110-3
AB  - The glucocorticoid-inducible transmembrane protein CD163 is a member of the
      scavenger receptor cysteine-rich (SRCR) family which is expressed exclusively on 
      human monocytes and macrophages. The expression of the protein is significantly
      downregulated in response to phorbol 12-myristate 13-acetate (PMA) by a yet
      unknown mechanism. We now demonstrate that PMA induces shedding of a soluble form
      of CD163 rather than internalization, revealing a novel regulatory mechanism for 
      a member of the SRCR family. Bisindolylmaleimide I was shown to inhibit phorbol
      ester-induced shedding, thus implying an involvement of protein kinase C (PKC).
      Furthermore, cleavage could be prevented by protease inhibitors. Therefore, we
      suggest that PMA-induced activation of PKC leads to protease-mediated shedding of
      CD163. These results indicate a specific release mechanism of soluble CD163 by
      human monocytes which could play an important role in modulating inflammatory
      processes.
CI  - Copyright 1999 Academic Press.
FAU - Droste, A
AU  - Droste A
AD  - Institute of Experimental Dermatology, Westfalische Wilhelms-University Munster, 
      Munster, Germany.
FAU - Sorg, C
AU  - Sorg C
FAU - Hogger, P
AU  - Hogger P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Androstadienes)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class B)
RN  - CUT2W21N7U (Fluticasone)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - MBK3OO5K8T (bisindolylmaleimide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Androstadienes/pharmacology
MH  - *Antigens, CD
MH  - Antigens, Differentiation, Myelomonocytic/*metabolism
MH  - Blotting, Western
MH  - Cell Membrane Permeability
MH  - Cells, Cultured
MH  - Cytoplasm/drug effects/metabolism
MH  - *Down-Regulation/drug effects
MH  - Flow Cytometry
MH  - Fluticasone
MH  - Humans
MH  - Indoles/pharmacology
MH  - Maleimides/pharmacology
MH  - *Membrane Proteins
MH  - Monocytes/drug effects/*metabolism
MH  - Protease Inhibitors/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - *Receptors, Cell Surface
MH  - Receptors, Immunologic/*metabolism
MH  - *Receptors, Lipoprotein
MH  - Receptors, Scavenger
MH  - Scavenger Receptors, Class B
MH  - Solubility/drug effects
MH  - Tetradecanoylphorbol Acetate/antagonists & inhibitors/pharmacology
MH  - Time Factors
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - S0006-291X(99)90294-2 [pii]
AID - 10.1006/bbrc.1999.0294 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Mar 5;256(1):110-3. doi: 10.1006/bbrc.1999.0294.