PMID- 10066421
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20211203
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 256
IP  - 1
DP  - 1999 Mar 5
TI  - Inactivation of mitogen-activated protein kinases by a mammalian
      tyrosine-specific phosphatase, PTPBR7.
PG  - 52-6
AB  - Mitogen-activated protein kinase (MAPK) is inactivated through dephosphorylation 
      of tyrosyl and threonyl regulatory sites. In yeast, both dual-specificity and
      tyrosine-specific phosphatases are involved in dephosphorylation. In mammals,
      however, no tyrosine-specific phosphatase has been identified molecularly to
      dephosphorylate MAPK in vivo. Recently, we and others have cloned a murine
      tyrosine-specific phosphatase, PTPBR7/PTP-SL, which is expressed predominantly in
      the brain. Here we report inactivation of the extracellular signal-regulated
      kinase (ERK) family MAPK by PTPBR7. PTPBR7 made complexes with ERK1/ERK2 in vivo 
      and dephosphorylated ERK1 in vitro. When overexpressed in mammalian cells,
      wild-type PTPBR7 suppressed the phosphorylation and activation of ERK by
      epidermal growth factor (EGF), nerve growth factor (NGF), and constitutively
      active MEK1, a mutant MAPK kinase. In contrast, catalytically inactive and
      ERK-binding-deficient mutants revealed little inhibition on the ERK cascade.
      These results indicate that PTPBR7 suppresses MAPK directly in vivo.
CI  - Copyright 1999 Academic Press.
FAU - Ogata, M
AU  - Ogata M
AD  - Biomedical Research Center, Osaka University Medical School C6, 2-2 Yamadaoka,
      Osaka, Suita, 565-0871, Japan. mogata@ongene.med.osaka-u.ac.jp
FAU - Oh-hora, M
AU  - Oh-hora M
FAU - Kosugi, A
AU  - Kosugi A
FAU - Hamaoka, T
AU  - Hamaoka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (MAP2K1 protein, human)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.1.3.48 (PTPRR protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptprr protein, rat)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 7)
SB  - IM
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*antagonists &
      inhibitors/genetics/metabolism
MH  - Catalytic Domain/genetics
MH  - Cell Line
MH  - Cell Membrane/enzymology
MH  - Cytosol/enzymology
MH  - Enzyme Activation/drug effects
MH  - Epidermal Growth Factor/pharmacology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Isoenzymes/genetics/metabolism
MH  - MAP Kinase Kinase 1
MH  - *Mitogen-Activated Protein Kinase Kinases
MH  - Mutation
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - PC12 Cells
MH  - Phosphorylation/drug effects
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Protein Tyrosine Phosphatases/genetics/*metabolism
MH  - Protein-Tyrosine Kinases/genetics/metabolism
MH  - Rats
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 7
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transfection
MH  - Yeasts/genetics/metabolism
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - S0006-291X(99)90278-4 [pii]
AID - 10.1006/bbrc.1999.0278 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Mar 5;256(1):52-6. doi: 10.1006/bbrc.1999.0278.