PMID- 10064895
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1437
IP  - 2
DP  - 1999 Feb 25
TI  - Identity of heart and liver L-3-hydroxyacyl coenzyme A dehydrogenase.
PG  - 119-23
AB  - Rat heart and liver cDNAs for precursor of L-3-hydroxyacyl-CoA dehydrogenase have
      been cloned and sequenced. The results indicate that these different rat organs
      express identical dehydrogenases. Furthermore, pig heart mRNA for
      L-3-hydroxyacyl-CoA dehydrogenase precursor was amplified by reverse
      transcription-polymerase chain reaction, and all the cDNA clones were found to
      encode a precursor of liver L-3-hydroxyacyl-CoA dehydrogenase (X.-Y. He, S.-Y.
      Yang, Biochim. Biophys. Acta 1392 (1998) 119-126) but not the well-documented
      heart form of the dehydrogenase (K.G. Bitar et al., FEBS Lett. 116 (1980)
      196-198). Sequencing data and other evidence establish that the pig, like the
      rat, has the same dehydrogenase in heart and liver. Since the size and structure 
      of pig heart L-3-hydroxyacyl-CoA dehydrogenase are identical to the pig liver
      dehydrogenase, reports that relied on the published sequence of the pig heart
      dehydrogenase need to be re-evaluated. For example, the signature pattern of the 
      L-3-hydroxyacyl-CoA dehydrogenase family is HXFXPX3MXLXE. Furthermore, the
      published crystal structure of the pig heart dehydrogenase that substantiated
      each subunit comprising 307 residues with a mercury-binding residue at position
      204 (J.J. Birktoft et al., Proc. Natl. Acad. Sci. U.S.A. 84 (1987) 8262-8266)
      must be re-examined in accordance with this revelation.
FAU - He, X Y
AU  - He XY
AD  - Department of Pharmacology, New York State Institute for Basic Research in
      Developmental Disabilities, Staten Island, NY 10314, USA.
FAU - Zhang, G
AU  - Zhang G
FAU - Blecha, F
AU  - Blecha F
FAU - Yang, S Y
AU  - Yang SY
LA  - eng
SI  - GENBANK/AF095449
GR  - DK47392/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (Protein Precursors)
RN  - EC 1.1.1.- (3-Hydroxyacyl CoA Dehydrogenases)
SB  - IM
MH  - 3-Hydroxyacyl CoA Dehydrogenases/biosynthesis/chemistry/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/biosynthesis
MH  - Liver/*enzymology
MH  - Mitochondria, Heart/enzymology
MH  - Molecular Sequence Data
MH  - Myocardium/*enzymology
MH  - Protein Precursors/biosynthesis/*genetics
MH  - Rats
MH  - Sequence Alignment
MH  - Swine
EDAT- 1999/03/05 00:00
MHDA- 1999/03/05 00:01
CRDT- 1999/03/05 00:00
PHST- 1999/03/05 00:00 [pubmed]
PHST- 1999/03/05 00:01 [medline]
PHST- 1999/03/05 00:00 [entrez]
AID - S1388-1981(98)00005-5 [pii]
AID - 10.1016/s1388-1981(98)00005-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Feb 25;1437(2):119-23. doi:
      10.1016/s1388-1981(98)00005-5.