PMID- 10064801
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 821
IP  - 1
DP  - 1999 Mar 6
TI  - Functional activation of EphA5 receptor does not promote cell proliferation in
      the aberrant EphA5 expressing human glioblastoma U-118 MG cell line.
PG  - 169-76
AB  - Eph receptors are a subfamily of receptor tyrosine kinases (RTKs), that are
      activated by ephrin ligands and appear to play important roles in axon guidance
      and cell migration during development of the nervous system. Over-expression or
      constitutive activation of Eph receptors has been linked with increased
      proliferation in various tumours. We have recently described lineage aberrant
      expression of EphA5 in primary human astrocytomas, glioblastomas and in the human
      glioblastoma U-118 MG cell line. A role for EphA5 expression in these tumours is 
      not apparent, and we have investigated the cellular effects of EphA5 activation
      using the human glioblastoma U-118 MG cell line as a model. Immunofluorescent
      staining demonstrated cell surface expression of EphA5. Activation of the EphA5
      receptor using an ephrin-A1 recombinant fusion protein resulted in tyrosine
      phosphorylation of EphA5 in a time-dependent manner. Exposure of U-118 MG
      glioblastoma cells to ephrin-A1 did not result in significant spontaneous or
      FCS-stimulated cell proliferation, though a marginal decrease was observed. This 
      is in converse to the effects of Eph activation in other tumour cell lines, and
      is the first study to investigate EphA5 in glioblastoma cell lines.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Bruce, V
AU  - Bruce V
AD  - Neurobiology Laboratory, Department of Research, University Hospital Basel,
      CH-4031, Basel, Switzerland.
FAU - Olivieri, G
AU  - Olivieri G
FAU - Eickelberg, O
AU  - Eickelberg O
FAU - Miescher, G C
AU  - Miescher GC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Ephrin-A1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, EphA5)
SB  - IM
MH  - Cell Division/physiology
MH  - Ephrin-A1
MH  - Glioblastoma/pathology/*physiopathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Membrane Proteins/physiology
MH  - Phosphorylation
MH  - Proteins/pharmacology
MH  - Receptor Protein-Tyrosine Kinases/*physiology
MH  - Receptor, EphA5
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1999/03/05 00:00
MHDA- 1999/03/05 00:01
CRDT- 1999/03/05 00:00
PHST- 1999/03/05 00:00 [pubmed]
PHST- 1999/03/05 00:01 [medline]
PHST- 1999/03/05 00:00 [entrez]
AID - S0006-8993(99)01112-9 [pii]
AID - 10.1016/s0006-8993(99)01112-9 [doi]
PST - ppublish
SO  - Brain Res. 1999 Mar 6;821(1):169-76. doi: 10.1016/s0006-8993(99)01112-9.