PMID- 10064583
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 5
DP  - 1999 Mar 1
TI  - The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and
      Eps15.
PG  - 1159-71
AB  - Clathrin-mediated endocytosis is a multistep process which requires interaction
      between a number of conserved proteins. We have cloned two mammalian genes which 
      code for a number of endocytic adaptor proteins. Two of these proteins, termed
      Ese1 and Ese2, contain two N-terminal EH domains, a central coiled-coil domain
      and five C-terminal SH3 domains. Ese1 is constitutively associated with Eps15
      proteins to form a complex with at least 14 protein-protein interaction surfaces.
      Yeast two-hybrid assays have revealed that Ese1 EH and SH3 domains bind epsin
      family proteins and dynamin, respectively. Overexpression of Ese1 is sufficient
      to block clathrin-mediated endocytosis in cultured cells, presumably through
      disruption of higher order protein complexes, which are assembled on the
      endogenous Ese1-Eps15 scaffold. The Ese1-Eps15 scaffold therefore links dynamin, 
      epsin and other endocytic pathway components.
FAU - Sengar, A S
AU  - Sengar AS
AD  - Programs of Cancer and Blood Research, and Developmental Biology, The Hospital
      for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada.
      segan@sickkids.on.ca
FAU - Wang, W
AU  - Wang W
FAU - Bishay, J
AU  - Bishay J
FAU - Cohen, S
AU  - Cohen S
FAU - Egan, S E
AU  - Egan SE
LA  - eng
SI  - GENBANK/AF132478
SI  - GENBANK/AF132479
SI  - GENBANK/AF132480
SI  - GENBANK/AF132481
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Clathrin)
RN  - 0 (Eps15 protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Itsn protein, mouse)
RN  - 0 (Itsn2 protein, mouse)
RN  - 0 (Neuropeptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (epsin)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - *Adaptor Proteins, Vesicular Transport
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Calcium-Binding Proteins/*metabolism
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Clathrin/metabolism
MH  - Cloning, Molecular
MH  - Dynamins
MH  - Endocytosis/*genetics
MH  - GTP Phosphohydrolases/*metabolism
MH  - Gene Expression Regulation/genetics
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neuropeptides/metabolism
MH  - Phosphoproteins/*metabolism
MH  - Protein Binding
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Transfection
MH  - *Vesicular Transport Proteins
MH  - src Homology Domains
PMC - PMC1171207
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
AID - 10.1093/emboj/18.5.1159 [doi]
PST - ppublish
SO  - EMBO J. 1999 Mar 1;18(5):1159-71. doi: 10.1093/emboj/18.5.1159.