PMID- 10064135
OWN - NLM
STAT- MEDLINE
DCOM- 19990423
LR  - 20061115
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 380
IP  - 1
DP  - 1999 Jan
TI  - Cytidine triphosphate synthase activity and mRNA expression in normal human blood
      cells.
PG  - 41-6
AB  - Cytidine triphosphate (CTP) synthase is one of the key enzymes in pyrimidine
      nucleotide anabolic pathways. The activity of this enzyme is elevated in various 
      malignancies including acute lymphocytic leukemia (ALL). In this study we
      investigated the activity of CTP synthase in various human blood cells isolated
      from healthy volunteers by density centrifugation and elutriation centrifugation.
      We also investigated the mRNA expression of CTP synthase in lymphocytes and
      monocytes. The highest activity of CTP synthase was found in thrombocytes (6.48
      nmol CTP x mg(-1) x h(-1)), followed by that of monocytes (2.23), lymphocytes
      (1.69), granulocytes (0.52) and erythrocytes (0.42). The activity of CTP synthase
      in whole blood samples was at an intermediate level (1.27). The mRNA expression
      of CTP synthase in monocytes was comparable to that observed in lymphocytes.
FAU - Verschuur, A C
AU  - Verschuur AC
AD  - Department of Pediatric Oncology, Academic Medical Centre, University of
      Amsterdam, The Netherlands.
FAU - Van Gennip, A H
AU  - Van Gennip AH
FAU - Muller, E J
AU  - Muller EJ
FAU - Voute, P A
AU  - Voute PA
FAU - Vreken, P
AU  - Vreken P
FAU - Van Kuilenburg, A B
AU  - Van Kuilenburg AB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (RNA, Messenger)
RN  - EC 6.3.- (Carbon-Nitrogen Ligases)
RN  - EC 6.3.4.2 (CTP synthetase)
SB  - IM
MH  - Blood Platelets/enzymology
MH  - Blotting, Northern
MH  - Carbon-Nitrogen Ligases/biosynthesis/*blood
MH  - Enzyme Activation
MH  - Erythrocytes/enzymology
MH  - Granulocytes/enzymology
MH  - Humans
MH  - Lymphocytes/enzymology
MH  - Monocytes/enzymology
MH  - RNA, Messenger/biosynthesis/*blood
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
AID - 10.1515/BC.1999.005 [doi]
PST - ppublish
SO  - Biol Chem. 1999 Jan;380(1):41-6. doi: 10.1515/BC.1999.005.