PMID- 10064132
OWN - NLM
STAT- MEDLINE
DCOM- 19990423
LR  - 20061115
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 380
IP  - 1
DP  - 1999 Jan
TI  - The human H2A and H2B histone gene complement.
PG  - 7-18
AB  - Sequences and expression patterns of newly isolated human histone H2A and H2B
      genes and the respective proteins were compared with previously isolated human
      H2A and H2B genes and proteins. Altogether, 15 human H2A genes and 17 human H2B
      genes have been identified. 14 of these are organized as H2A/H2B gene pairs,
      while one H2A gene and three H2B genes are solitary genes. Two H2A genes and two 
      H2B genes turned outto be pseudogenes. The 13 H2A genes code for at least 6
      different amino acid sequences, and the 15 H2B genes encode 11 different H2B
      isoforms. Each H2A/H2B gene pair is controlled by a divergent promoter spanning
      300 to 330 nucleotides between the coding regions of the two genes. The highly
      conserved divergent H2A/H2B promoters can be classified in two groups based on
      the patterns of consensus sequence elements. Group I promoters contain a TATA box
      for each gene, two Oct-1 factor binding sites, and three CCAAT boxes. Group II
      promoters contain the same elements as group I promoters and an additional CCAAT 
      box, a binding motif for E2F and adjacent a highly conserved octanucleotide
      (CACAGCTT) that has not been described so far. Five of the 6 gene pairs and 4
      solitary genes with group I promoters are localized in the large histone gene
      cluster at 6p21.3-6p22, and one gene pair is located at 1q21. All group II
      promoter associated genes are contained within the histone gene subcluster at
      D6S105, which is located at a distance of about 2 Mb from the major subcluster at
      6p21.3-6p22 containing histone genes with group I promoters. Almost all group II 
      H2A genes encode identical amino acid sequences, whereas group I H2A gene
      products vary at several positions. Using human cell lines, we have analyzed the 
      expression patterns of functional human H2A/H2B gene pairs organized within the
      two histone gene clusters on the short arm of chromosome 6. The genes show
      varying expression patterns in different tumor cell lines.
FAU - Albig, W
AU  - Albig W
AD  - Institut fur Biochemie und Molekulare Zellbiologie, Universitat Gottingen,
      Germany.
FAU - Trappe, R
AU  - Trappe R
FAU - Kardalinou, E
AU  - Kardalinou E
FAU - Eick, S
AU  - Eick S
FAU - Doenecke, D
AU  - Doenecke D
LA  - eng
SI  - GENBANK/Z83336
SI  - GENBANK/Z83736
SI  - GENBANK/Z83738
SI  - GENBANK/Z83739
SI  - GENBANK/Z83740
SI  - GENBANK/Z83742
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (Histones)
SB  - IM
MH  - Amino Acid Sequence
MH  - Gene Expression
MH  - *Genes
MH  - Histones/biosynthesis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
AID - 10.1515/BC.1999.002 [doi]
PST - ppublish
SO  - Biol Chem. 1999 Jan;380(1):7-18. doi: 10.1515/BC.1999.002.