PMID- 10064083
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20180425
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Feb
TI  - Identification of destabilizing residues in HLA class II-selected bacteriophage
      display libraries edited by HLA-DM.
PG  - 660-8
AB  - HLA-DM (DM) functions as a peptide editor by catalyzing the release of class
      II-associated invariant chain peptides (CLIP) and other unstable peptides, thus
      supporting the formation of stable class II-peptide complexes for presentation.
      To investigate the general features that determine the DM susceptibility of
      HLA-DR1/peptide complexes, we generated a large DM-sensitive peptide repertoire
      from an M13 bacteriophage display library using a novel double selection
      protocol: we selected bacteriophage capable of binding to DR1 molecules and,
      subsequently, we enriched DR1-bound bacteriophage susceptible to elution by
      purified DM molecules. Sequence and mutational analyses of the DR1/DM
      double-selected peptides revealed that the amino acids Gly and Pro play a
      destabilizing role in the dissociation kinetics of DR1 ligands. This observation 
      was confirmed also in natural peptide sequences such as CLIP 89-101, HA 307-319
      and bovine collagen II (CII) 261-273. Our results demonstrate that DM
      susceptibility does not only depend on the number and nature of anchor residues, 
      or the peptide length. Instead, less obvious sequence characteristics play a
      major role in the DM editing process and ultimately in the composition of peptide
      repertoires presented to T cells.
FAU - Raddrizzani, L
AU  - Raddrizzani L
AD  - Roche Milano Ricerche, Milan, Italy.
FAU - Bono, E
AU  - Bono E
FAU - Vogt, A B
AU  - Vogt AB
FAU - Kropshofer, H
AU  - Kropshofer H
FAU - Gallazzi, F
AU  - Gallazzi F
FAU - Sturniolo, T
AU  - Sturniolo T
FAU - Hammerling, G J
AU  - Hammerling GJ
FAU - Sinigaglia, F
AU  - Sinigaglia F
FAU - Hammer, J
AU  - Hammer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (H2-M antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptide Library)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation/genetics/*immunology
MH  - Bacteriophages
MH  - Cattle
MH  - HLA-D Antigens/*genetics/*immunology
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Peptide Library
MH  - Sequence Analysis
EDAT- 1999/03/04 03:38
MHDA- 2000/06/20 09:00
CRDT- 1999/03/04 03:38
PHST- 1999/03/04 03:38 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/03/04 03:38 [entrez]
AID - 10.1002/(SICI)1521-4141(199902)29:02<660::AID-IMMU660>3.0.CO;2-I [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Feb;29(2):660-8. doi:
      10.1002/(SICI)1521-4141(199902)29:02<660::AID-IMMU660>3.0.CO;2-I.