PMID- 10064080
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20180425
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Feb
TI  - Selective expression of liver and activation-regulated chemokine (LARC) in
      intestinal epithelium in mice and humans.
PG  - 633-42
AB  - The liver and activation-regulated chemokine (LARC), also termed MIP-3alpha and
      Exodus, is a novel human CC chemokine with a selective chemotactic activity for
      lymphocytes and dendritic cells. Here we describe genomic and cDNA clones
      encoding the murine orthologue of LARC (mLARC). The gene consists of four exons
      and three introns. The 5'-noncoding region of about 400 bp contains typical TATA 
      and CAAT boxes but no other potential regulatory elements so far described. The
      cDNA encodes a CC chemokine of 97 amino acid residues with the highest homology
      to human LARC (64% amino acid identity). The 3'-noncoding region contains as many
      as five potential mRNA destabilization signals. mLARC was strongly and
      transiently induced in the murine monocytoid cell line J774 by lipopolysaccharide
      (LPS) but not by cytokines such as TNF-alpha, IFN-gamma, IL-1beta or IL-4. In
      normal mice, mLARC mRNA was expressed selectively in intestinal tissues such as
      small intestine and colon. Upon treatment with LPS, mLARC expression was enhanced
      in intestinal tissues and induced in some lymphoid tissues such as lymph nodes.
      Because of alternative splicing, there are two types of transcripts encoding
      mLARC and its variant mLARCvar with and without an N-terminal alanine in the
      mature protein, respectively. Both types of transcripts appeared to be expressed 
      in various mouse tissues. In situ hybridization revealed that epithelial cells of
      intestinal tissues, especially those lining lymphoid follicles, expressed mLARC. 
      Localization of LARC mRNA in epithelial cells was also demonstrated in a human
      appendix. Furthermore, mLARC was efficiently chemotactic for cells such as
      gammadelta type T cells in intestinal epithelium and naive B cells in Peyer's
      patches. Thus, in both humans and mice, LARC may be physiologically involved in
      formation and function of the mucosal lymphoid tissues by attracting lymphocytes 
      and dendritic cells toward epithelial cells.
FAU - Tanaka, Y
AU  - Tanaka Y
AD  - Shionogi Institute for Medical Science, Osaka, Osaka-Sayama, Japan.
FAU - Imai, T
AU  - Imai T
FAU - Baba, M
AU  - Baba M
FAU - Ishikawa, I
AU  - Ishikawa I
FAU - Uehira, M
AU  - Uehira M
FAU - Nomiyama, H
AU  - Nomiyama H
FAU - Yoshie, O
AU  - Yoshie O
LA  - eng
SI  - GENBANK/AB015136
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (CCL20 protein, human)
RN  - 0 (CCR6 protein, human)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Chemokines, CC)
RN  - 0 (DNA, Complementary)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR6)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chemokine CCL20
MH  - Chemokines, CC/*biosynthesis/*immunology
MH  - DNA, Complementary/analysis
MH  - Humans
MH  - Immunity, Mucosal
MH  - In Situ Hybridization
MH  - Intestinal Mucosa/*immunology/metabolism
MH  - *Macrophage Inflammatory Proteins
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Receptors, CCR6
MH  - *Receptors, Chemokine
EDAT- 1999/03/04 03:38
MHDA- 2000/06/20 09:00
CRDT- 1999/03/04 03:38
PHST- 1999/03/04 03:38 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/03/04 03:38 [entrez]
AID - 10.1002/(SICI)1521-4141(199902)29:02<633::AID-IMMU633>3.0.CO;2-I [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Feb;29(2):633-42. doi:
      10.1002/(SICI)1521-4141(199902)29:02<633::AID-IMMU633>3.0.CO;2-I.