PMID- 10064069
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20180425
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Feb
TI  - Cell surface expression of HLA-E: interaction with human beta2-microglobulin and 
      allelic differences.
PG  - 537-47
AB  - The formation of a trimeric complex composed of MHC class I heavy chain,
      beta2-microglobulin (beta2m) and peptide ligand is a prerequisite for its
      efficient transport to the cell surface. We have previously demonstrated impaired
      intracellular transport of the human class Ib molecule HLA-E in mouse myeloma X63
      cells cotransfected with the genes for HLA-E and human beta2m (hbeta2m), which is
      most likely attributable to inefficient intracellular peptide loading of the
      HLA-E molecule. Here we demonstrate that cell surface expression of HLA-E in
      mouse cells strictly depends on the coexpression of hbeta2m and that soluble
      empty complexes of HLA-E and hbeta2m display a low degree of thermostability.
      Both observations imply that low affinity interaction of HLA-E with beta2m
      accounts to a considerable extent for the observed low degree of peptide uptake
      in the endoplasmic reticulum. Moreover, we show that the only allelic variation
      present in the caucasoid population located at amino acid position 107 (Gly or
      Arg) greatly affects intracellular transport and cell surface expression upon
      transfection of the respective alleles into mouse cells. No obvious difference
      was found with regard to the sequence of the peptide ligand.
FAU - Ulbrecht, M
AU  - Ulbrecht M
AD  - Institut fur Anthropologie und Humangenetik, Ludwig-Maximillians-Universitat
      Munchen, Germany.
FAU - Couturier, A
AU  - Couturier A
FAU - Martinozzi, S
AU  - Martinozzi S
FAU - Pla, M
AU  - Pla M
FAU - Srivastava, R
AU  - Srivastava R
FAU - Peterson, P A
AU  - Peterson PA
FAU - Weiss, E H
AU  - Weiss EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-E antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Antigen Presentation/genetics/*immunology
MH  - Gene Expression Regulation/immunology
MH  - HLA Antigens/genetics/*immunology
MH  - Histocompatibility Antigens Class I/genetics/*immunology
MH  - Humans
MH  - Mice
MH  - Multiple Myeloma/genetics/immunology
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - beta 2-Microglobulin/genetics/*immunology
EDAT- 1999/03/04 03:38
MHDA- 2000/06/20 09:00
CRDT- 1999/03/04 03:38
PHST- 1999/03/04 03:38 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/03/04 03:38 [entrez]
AID - 10.1002/(SICI)1521-4141(199902)29:02<537::AID-IMMU537>3.0.CO;2-6 [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Feb;29(2):537-47. doi:
      10.1002/(SICI)1521-4141(199902)29:02<537::AID-IMMU537>3.0.CO;2-6.