PMID- 10053003
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20211203
IS  - 0002-9297 (Print)
IS  - 0002-9297 (Linking)
VI  - 64
IP  - 3
DP  - 1999 Mar
TI  - Human molybdopterin synthase gene: identification of a bicistronic transcript
      with overlapping reading frames.
PG  - 698-705
AB  - A universal molybdenum-containing cofactor (MoCo) is essential for the activity
      of all human molybdoenzymes, including sulphite oxidase. The free cofactor is
      highly unstable, and all organisms share a similar biosynthetic pathway. The
      involved enzymes exhibit homologies, even between bacteria and humans. We have
      exploited these homologies to isolate a cDNA for the heterodimeric molybdopterin 
      (MPT)-synthase. This enzyme is necessary for the conversion of an unstable
      precursor into molybdopterin, the organic moiety of MoCo. The corresponding
      transcript shows a bicistronic structure, encoding the small and large subunits
      of the MPT-synthase in two different open reading frames (ORFs) that overlap by
      77 nucleotides. In various human tissues, only one size of mRNA coinciding with
      the bicistronic transcript was detected. In vitro translation and mutagenesis
      experiments demonstrated that each ORF is translated independently, leading to
      the synthesis of a 10-kDa protein and a 21-kDa protein for the small and large
      subunits, respectively, and indicated that the 3'-proximal ORF of the bicistronic
      transcript is translated by leaky scanning.
FAU - Stallmeyer, B
AU  - Stallmeyer B
AD  - Botanisches Institut der Technischen Universitat, Braunschweig, Humboldtstr. 1,
      D-38106 Braunschweig, Germany.
FAU - Drugeon, G
AU  - Drugeon G
FAU - Reiss, J
AU  - Reiss J
FAU - Haenni, A L
AU  - Haenni AL
FAU - Mendel, R R
AU  - Mendel RR
LA  - eng
SI  - GENBANK/AF091871
SI  - GENBANK/AF091872
SI  - GENBANK/D64003
SI  - GENBANK/U21322
SI  - GENBANK/U29587
SI  - GENBANK/U88180
SI  - GENBANK/X70420
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Hum Genet
JT  - American journal of human genetics
JID - 0370475
RN  - 0 (Coenzymes)
RN  - 0 (Metalloproteins)
RN  - 0 (Molybdenum Cofactors)
RN  - 0 (Pteridines)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - ATN6EG42UQ (molybdenum cofactor)
RN  - EC 2.8.1.- (Sulfurtransferases)
RN  - EC 2.8.1.- (molybdopterin synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - *Coenzymes
MH  - Escherichia coli/genetics
MH  - Gene Library
MH  - Genes, Overlapping
MH  - Guanosine Triphosphate/metabolism
MH  - Humans
MH  - Liver/metabolism
MH  - Metalloproteins/metabolism
MH  - Molecular Sequence Data
MH  - Molybdenum Cofactors
MH  - Mutagenesis, Insertional
MH  - Protein Biosynthesis/physiology
MH  - Pteridines/metabolism
MH  - Rabbits
MH  - Reading Frames/genetics
MH  - Reticulocytes/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sulfurtransferases/*genetics
PMC - PMC1377786
EDAT- 1999/03/03 03:04
MHDA- 2000/03/21 09:00
CRDT- 1999/03/03 03:04
PHST- 1999/03/03 03:04 [pubmed]
PHST- 2000/03/21 09:00 [medline]
PHST- 1999/03/03 03:04 [entrez]
AID - S0002-9297(07)61706-6 [pii]
AID - 10.1086/302295 [doi]
PST - ppublish
SO  - Am J Hum Genet. 1999 Mar;64(3):698-705. doi: 10.1086/302295.