PMID- 10052594
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20191024
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 15
IP  - 9
DP  - 1998 Sep
TI  - N-glycan structures of a recombinant mouse soluble Fcgamma receptor II.
PG  - 905-14
AB  - N-glycans of a recombinant mouse soluble Fcgamma receptor II (sFcgammaRII)
      expressed in baby hamster kidney cells were released from glycopeptides by
      digestion with glycoamidase A (from sweet almond), and the reducing ends of the
      oligosaccharides were reductively aminated with 2-aminopyridine. The derivatized 
      N-glycans were separated and structurally identified by a three-dimensional
      high-performance liquid chromatography (HPLC) mapping technique on three kinds of
      HPLC columns [Takahashi, et al. (1995) Anal. Biochem. 226:139-46]. Eighteen
      different major N-glycan structures were identified, of which six were neutral
      (45%), five mono-sialyl (49%), one di-sialyl (4.6%), five tri-sialyl (1.1%), and 
      one tetra-sialyl (0.3%). All N-glycan structures determined were complex type
      with fucosylation at the N-acetylglucosamine residue of the reducing end, and
      N-acetylneuraminic acid, when present, was alpha-(2,3)-linked. The existence of a
      unique structure containing both N-acetylgalactosamine and
      alpha-(2,3)-N-acetylneuraminic acid residues at the reducing ends, as below, was 
      confirmed by MALDI-TOF mass spectrometry. Carbohydrate sequence [see text]
FAU - Takahashi, N
AU  - Takahashi N
AD  - GlycoLab, Nakano Vinegar Co., Ltd., Handa-City Aichi, Japan.
FAU - Yamada, W
AU  - Yamada W
FAU - Masuda, K
AU  - Masuda K
FAU - Araki, H
AU  - Araki H
FAU - Tsukamoto, Y
AU  - Tsukamoto Y
FAU - Galinha, A
AU  - Galinha A
FAU - Sautes, C
AU  - Sautes C
FAU - Kato, K
AU  - Kato K
FAU - Shimada, I
AU  - Shimada I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Glycoproteins/chemistry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oligosaccharides/chemistry
MH  - Polysaccharides/*chemistry
MH  - Receptors, IgG/*chemistry/genetics
MH  - Recombinant Proteins/chemistry
MH  - Solubility
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1023/a:1006915200989 [doi]
PST - ppublish
SO  - Glycoconj J. 1998 Sep;15(9):905-14. doi: 10.1023/a:1006915200989.