PMID- 10052459
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20210930
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 4
DP  - 1999 Feb 19
TI  - Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled
      inheritance of chromatin.
PG  - 575-85
AB  - Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically
      determined chromosomal states in vivo and promotes assembly of chromatin during
      DNA replication in vitro. Herein, we demonstrate that after DNA replication,
      replicated, but not unreplicated, DNA is also competent for CAF-1-dependent
      chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA
      polymerase clamp, is a component of the replication-dependent marking of DNA for 
      chromatin assembly. The clamp loader, replication factor C (RFC), can reverse
      this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150,
      the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA
      replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to
      chromatin assembly and the inheritance of epigenetic chromosome states.
FAU - Shibahara, K
AU  - Shibahara K
AD  - Cold Spring Harbor Laboratory, New York 11724, USA.
FAU - Stillman, B
AU  - Stillman B
LA  - eng
GR  - CA13106/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Autoantibodies)
RN  - 0 (BCL2-related protein A1)
RN  - 0 (CHAF1B protein, human)
RN  - 0 (CNOT8 protein, human)
RN  - 0 (Chromatin)
RN  - 0 (Chromatin Assembly Factor-1)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Superhelical)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MATA1 protein, S cerevisiae)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.4.- (Replication Protein C)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Autoantibodies/pharmacology
MH  - Cell Division/genetics
MH  - Chromatin/*genetics/metabolism
MH  - Chromatin Assembly Factor-1
MH  - *Chromosomal Proteins, Non-Histone
MH  - DNA Replication/drug effects/*genetics
MH  - DNA, Superhelical/drug effects
MH  - DNA-Binding Proteins/*genetics/metabolism/pharmacology
MH  - HeLa Cells
MH  - *Homeodomain Proteins
MH  - Humans
MH  - Minor Histocompatibility Antigens
MH  - Proliferating Cell Nuclear Antigen/*genetics/immunology/metabolism
MH  - Protein Binding/genetics
MH  - *Proto-Oncogene Proteins c-bcl-2
MH  - Replication Protein C
MH  - *Repressor Proteins
MH  - *Saccharomyces cerevisiae Proteins
MH  - Simian virus 40/genetics
MH  - Temperature
MH  - Transcription Factors
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - S0092-8674(00)80661-3 [pii]
AID - 10.1016/s0092-8674(00)80661-3 [doi]
PST - ppublish
SO  - Cell. 1999 Feb 19;96(4):575-85. doi: 10.1016/s0092-8674(00)80661-3.