PMID- 10052452
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 4
DP  - 1999 Feb 19
TI  - Coupling of coat assembly and vesicle budding to packaging of putative cargo
      receptors.
PG  - 495-506
AB  - COPI-coated vesicle budding from lipid bilayers whose composition resembles
      mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of
      putative cargo receptors (p24 family proteins) or membrane cargo proteins
      (containing the KKXX retrieval signal) emanating from the bilayer surface.
      Liposome-derived COPI-coated vesicles are similar to their native counterparts
      with respect to diameter, buoyant density, morphology, and the requirement for an
      elevated temperature for budding. These results suggest that a bivalent
      interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic
      tails of cargo or putative cargo receptors is the molecular basis of COPI coat
      assembly and provide a simple mechanism to couple uptake of cargo to transport
      vesicle formation.
FAU - Bremser, M
AU  - Bremser M
AD  - Biochemie-Zentrum Heidelberg, University of Heidelberg, Germany.
FAU - Nickel, W
AU  - Nickel W
FAU - Schweikert, M
AU  - Schweikert M
FAU - Ravazzola, M
AU  - Ravazzola M
FAU - Amherdt, M
AU  - Amherdt M
FAU - Hughes, C A
AU  - Hughes CA
FAU - Sollner, T H
AU  - Sollner TH
FAU - Rothman, J E
AU  - Rothman JE
FAU - Wieland, F T
AU  - Wieland FT
LA  - eng
GR  - GM19079-01/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Carrier Proteins)
RN  - 0 (Coatomer Protein)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Liposomes)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Fragments)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport/physiology
MH  - Carrier Proteins/metabolism
MH  - Cell Compartmentation/physiology
MH  - Coatomer Protein
MH  - Cytoplasm/chemistry/metabolism
MH  - Cytoplasmic Granules/chemistry/*metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Triphosphate/metabolism
MH  - Humans
MH  - Lipid Bilayers/chemistry/*metabolism
MH  - Liposomes/chemistry/metabolism
MH  - Membrane Proteins/analysis/chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Peptide Fragments/analysis/metabolism
MH  - Protein Binding/drug effects/physiology
MH  - Rabbits
MH  - Temperature
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - S0092-8674(00)80654-6 [pii]
AID - 10.1016/s0092-8674(00)80654-6 [doi]
PST - ppublish
SO  - Cell. 1999 Feb 19;96(4):495-506. doi: 10.1016/s0092-8674(00)80654-6.