PMID- 10051678
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 2
TI  - Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates 
      transcription in response to hypertonicity.
PG  - 2538-42
AB  - Hypertonicity (most often present as high salinity) is stressful to the cells of 
      virtually all organisms. Cells survive in a hypertonic environment by increasing 
      the transcription of genes whose products catalyze cellular accumulation of
      compatible osmolytes. In mammals, the kidney medulla is normally hypertonic
      because of the urinary concentrating mechanism. Cellular accumulation of
      compatible osmolytes in the renal medulla is catalyzed by the sodium/myo-inositol
      cotransporter (SMIT), the sodium/chloride/betaine cotransporter, and aldose
      reductase (synthesis of sorbitol). The importance of compatible osmolytes is
      underscored by the necrotic injury of the renal medulla and subsequent renal
      failure that results from the inhibition of SMIT in vivo by administration of a
      specific inhibitor. Tonicity-responsive enhancers (TonE) play a key role in
      hypertonicity-induced transcriptional stimulation of SMIT,
      sodium/chloride/betaine cotransporter, and aldose reductase. We report the cDNA
      cloning of human TonE binding protein (TonEBP), a transcription factor that
      stimulates transcription through its binding to TonE sequences via a Rel-like DNA
      binding domain. Western blot and immunohistochemical analyses of cells cultured
      in hypertonic medium reveal that exposure to hypertonicity elicits slow
      activation of TonEBP, which is the result of an increase in TonEBP amount and
      translocation to the nucleus.
FAU - Miyakawa, H
AU  - Miyakawa H
AD  - Division of Nephrology, Johns Hopkins School of Medicine, 963 Ross Building, 720 
      Rutland Avenue, Baltimore, MD 21205, USA.
FAU - Woo, S K
AU  - Woo SK
FAU - Dahl, S C
AU  - Dahl SC
FAU - Handler, J S
AU  - Handler JS
FAU - Kwon, H M
AU  - Kwon HM
LA  - eng
SI  - GENBANK/AF089824
GR  - P01 DK44484/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hypertonic Solutions)
RN  - 0 (NFAT5 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saline Solution, Hypertonic)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
CIN - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1814-6. PMID: 10051549
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Dogs
MH  - Enhancer Elements, Genetic
MH  - *Gene Expression Regulation
MH  - Gene Library
MH  - HeLa Cells
MH  - Humans
MH  - Hypertonic Solutions
MH  - Kidney/metabolism
MH  - Kidney Medulla/*physiology
MH  - Mammals
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Saline Solution, Hypertonic
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Trans-Activators/chemistry/*genetics/*metabolism
MH  - Transcription Factors
MH  - *Transcription, Genetic
MH  - Transfection
PMC - PMC26820
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1073/pnas.96.5.2538 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2538-42. doi: 10.1073/pnas.96.5.2538.