PMID- 10051670
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 2
TI  - Cloning, expression, and genetic mapping of Sema W, a member of the semaphorin
      family.
PG  - 2491-6
AB  - The semaphorins comprise a large family of membrane-bound and secreted proteins, 
      some of which have been shown to function in axon guidance. We have cloned a
      transmembrane semaphorin, Sema W, that belongs to the class IV subgroup of the
      semaphorin family. The mouse and rat forms of Sema W show 97% amino acid sequence
      identity with each other, and each shows about 91% identity with the human form. 
      The gene for Sema W is divided into 15 exons, up to 4 of which are absent in the 
      human cDNAs that we sequenced. Unlike many other semaphorins, Sema W is expressed
      at low levels in the developing embryo but was found to be expressed at high
      levels in the adult central nervous system and lung. Functional studies with
      purified membrane fractions from COS7 cells transfected with a Sema W expression 
      plasmid showed that Sema W has growth-cone collapse activity against retinal
      ganglion-cell axons, indicating that vertebrate transmembrane semaphorins, like
      secreted semaphorins, can collapse growth cones. Genetic mapping of human SEMAW
      with human/hamster radiation hybrids localized the gene to chromosome 2p13.
      Genetic mapping of mouse Semaw with mouse/hamster radiation hybrids localized the
      gene to chromosome 6, and physical mapping placed the gene on bacteria artificial
      chromosomes carrying microsatellite markers D6Mit70 and D6Mit189. This
      localization places Semaw within the locus for motor neuron degeneration 2,
      making it an attractive candidate gene for this disease.
FAU - Encinas, J A
AU  - Encinas JA
AD  - Sumitomo Pharmaceuticals Research Center, 3-1-98 Kasugade-Naka, Konohana, Osaka
      554-0022, Japan.
FAU - Kikuchi, K
AU  - Kikuchi K
FAU - Chedotal, A
AU  - Chedotal A
FAU - de Castro, F
AU  - de Castro F
FAU - Goodman, C S
AU  - Goodman CS
FAU - Kimura, T
AU  - Kimura T
LA  - eng
SI  - GENBANK/AB002563
SI  - GENBANK/AB021291
SI  - GENBANK/AB021292
SI  - GENBANK/AB022311
SI  - GENBANK/AB022312
SI  - GENBANK/AB022313
SI  - GENBANK/AB022314
SI  - GENBANK/AB022315
SI  - GENBANK/AB022316
SI  - GENBANK/AB022317
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SEMA4F protein, human)
RN  - 0 (Sema4f protein, mouse)
RN  - 0 (Sema4f protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 2
MH  - Cloning, Molecular
MH  - Databases as Topic
MH  - Embryo, Mammalian
MH  - Expressed Sequence Tags
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Situ Hybridization
MH  - Membrane Proteins/biosynthesis/chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/biosynthesis/chemistry/*genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC26812
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1073/pnas.96.5.2491 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2491-6. doi: 10.1073/pnas.96.5.2491.