PMID- 10051605
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 2
TI  - The small GTPase RalA targets filamin to induce filopodia.
PG  - 2122-8
AB  - The Ras-related small GTPases Rac, Rho, Cdc42, and RalA bind filamin, an actin
      filament-crosslinking protein that also links membrane and other intracellular
      proteins to actin. Of these GTPases only RalA binds filamin in a GTP-specific
      manner, and GTP-RalA elicits actin-rich filopods on surfaces of Swiss 3T3 cells
      and recruits filamin into the filopodial cytoskeleton. Either a dominant negative
      RalA construct or the RalA-binding domain of filamin 1 specifically block
      Cdc42-induced filopod formation, but a Cdc42 inhibitor does not impair RalA's
      effects, which, unlike Cdc42, are Rac independent. RalA does not generate
      filopodia in filamin-deficient human melanoma cells, whereas transfection of
      filamin 1 restores the functional response. RalA therefore is a downstream
      intermediate in Cdc42-mediated filopod production and uses filamin in this
      pathway.
FAU - Ohta, Y
AU  - Ohta Y
AD  - Division of Biochemistry and Cellular Biology, National Institute of
      Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi,
      Kodaira, Tokyo 187, Japan. ohta@ncnaxp.ncnp.go.jp
FAU - Suzuki, N
AU  - Suzuki N
FAU - Nakamura, S
AU  - Nakamura S
FAU - Hartwig, J H
AU  - Hartwig JH
FAU - Stossel, T P
AU  - Stossel TP
LA  - eng
GR  - R01 HL054145/HL/NHLBI NIH HHS/United States
GR  - HL54145/HL/NHLBI NIH HHS/United States
GR  - HL56949/HL/NHLBI NIH HHS/United States
GR  - R01 HL056252/HL/NHLBI NIH HHS/United States
GR  - HL56252/HL/NHLBI NIH HHS/United States
GR  - P01 HL056949/HL/NHLBI NIH HHS/United States
GR  - R01 HL019429/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Contractile Proteins)
RN  - 0 (Filamins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (RALA protein, human)
RN  - EC 3.6.1.- (Rala protein, mouse)
RN  - EC 3.6.5.2 (ral GTP-Binding Proteins)
SB  - IM
MH  - 3T3 Cells
MH  - Actins/metabolism
MH  - Animals
MH  - COS Cells
MH  - Carrier Proteins/metabolism
MH  - Cloning, Molecular
MH  - Contractile Proteins/*metabolism
MH  - Cytoskeleton/physiology/ultrastructure
MH  - Escherichia coli
MH  - Filamins
MH  - GTP Phosphohydrolases/genetics/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Microfilament Proteins/*metabolism
MH  - Microscopy, Video
MH  - Pseudopodia/*physiology/ultrastructure
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Recombinant Proteins/metabolism
MH  - Time Factors
MH  - Transfection
MH  - *ral GTP-Binding Proteins
PMC - PMC26747
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1073/pnas.96.5.2122 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2122-8. doi: 10.1073/pnas.96.5.2122.