PMID- 10051575
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 2
TI  - The GTPase activating factor for transducin in rod photoreceptors is the complex 
      between RGS9 and type 5 G protein beta subunit.
PG  - 1947-52
AB  - Proteins of the regulators of G protein signaling (RGS) family modulate the
      duration of intracellular signaling by stimulating the GTPase activity of G
      protein alpha subunits. It has been established that the ninth member of the RGS 
      family (RGS9) participates in accelerating the GTPase activity of the
      photoreceptor-specific G protein, transducin. This process is essential for
      timely inactivation of the phototransduction cascade during the recovery from a
      photoresponse. Here we report that functionally active RGS9 from vertebrate
      photoreceptors exists as a tight complex with the long splice variant of the G
      protein beta subunit (Gbeta5L). RGS9 and Gbeta5L also form a complex when
      coexpressed in cell culture. Our data are consistent with the recent observation 
      that several RGS proteins, including RGS9, contain G protein gamma-subunit like
      domain that can mediate their association with Gbeta5 (Snow, B. E., Krumins, A.
      M., Brothers, G. M., Lee, S. F., Wall, M. A., Chung, S., Mangion, J., Arya, S.,
      Gilman, A. G. & Siderovski, D. P. (1998) Proc. Natl. Acad. Sci. USA 95,
      13307-13312). We report an example of such a complex whose cellular localization 
      and function are clearly defined.
FAU - Makino, E R
AU  - Makino ER
AD  - Department of Ophthalmology, Harvard Medical School, and the Massachusetts Eye
      and Ear Infirmary, Boston, MA 02114, USA.
FAU - Handy, J W
AU  - Handy JW
FAU - Li, T
AU  - Li T
FAU - Arshavsky, V Y
AU  - Arshavsky VY
LA  - eng
GR  - R01 EY010336/EY/NEI NIH HHS/United States
GR  - EY 10336/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Proteins)
RN  - 0 (ras GTPase-Activating Proteins)
RN  - 9009-81-8 (Rhodopsin)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.1 (Transducin)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies
MH  - Epitopes/chemistry
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTP-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - GTPase-Activating Proteins
MH  - Macromolecular Substances
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Proteins/*chemistry/isolation & purification/*metabolism
MH  - Rhodopsin/metabolism
MH  - Rod Cell Outer Segment/*metabolism
MH  - Transducin/isolation & purification/*metabolism
MH  - Vertebrates
MH  - ras GTPase-Activating Proteins
MH  - ras Proteins/metabolism
PMC - PMC26717
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1073/pnas.96.5.1947 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1947-52. doi: 10.1073/pnas.96.5.1947.