PMID- 10051570
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 2
TI  - Nuclear foci of mammalian recombination proteins are located at single-stranded
      DNA regions formed after DNA damage.
PG  - 1921-6
AB  - A sensitive and rapid in situ method was developed to visualize sites of
      single-stranded (ss) DNA in cultured cells and in experimental test animals.
      Anti-bromodeoxyuridine antibody recognizes the halogenated base analog
      incorporated into chromosomal DNA only when substituted DNA is in the single
      strand form. After treatment of cells with DNA-damaging agents or gamma
      irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 
      60 min. The mammalian recombination protein Rad51 and the replication protein A
      then accumulate at sites of ssDNA and form foci, suggesting that these are sites 
      of recombinational DNA repair.
FAU - Raderschall, E
AU  - Raderschall E
AD  - Max Planck Institute of Molecular Genetics, Ihnestrasse 73, 14195 Berlin,
      Germany.
FAU - Golub, E I
AU  - Golub EI
FAU - Haaf, T
AU  - Haaf T
LA  - eng
GR  - R37-GM33504/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Chromatin)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 50SG953SK6 (Mitomycin)
RN  - 6PLQ3CP4P3 (Etoposide)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Animals
MH  - *Cell Cycle
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - Chromatin/physiology/radiation effects
MH  - Cycloheximide/pharmacology
MH  - *DNA Damage
MH  - DNA, Single-Stranded/*analysis
MH  - Dactinomycin/pharmacology
MH  - Etoposide/toxicity
MH  - Fibroblasts
MH  - Gamma Rays
MH  - Humans
MH  - Kinetics
MH  - Mammals
MH  - Meiosis
MH  - Mitomycin/toxicity
MH  - Nuclear Proteins/drug effects/*metabolism/radiation effects
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Sensitivity and Specificity
MH  - Xeroderma Pigmentosum
PMC - PMC26712
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1073/pnas.96.5.1921 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1921-6. doi: 10.1073/pnas.96.5.1921.