PMID- 10051439
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 338 ( Pt 3)
DP  - 1999 Mar 15
TI  - Stress- and cell type-dependent regulation of transfected c-Jun N-terminal kinase
      and mitogen-activated protein kinase kinase isoforms.
PG  - 681-6
AB  - The cJun N-terminal kinases (JNKs) are encoded by three genes generating ten
      protein kinase polypeptides and are activated in settings of cell stress,
      mitogenesis, differentiation and morphogenesis. The specific role of the JNK
      family members in these diverse cell programmes is largely undefined. In this
      study, we tested the hypothesis that individual JNK isoforms would exhibit
      distinct patterns of regulation within cells. The cDNAs encoding five
      haemagglutinin (HA)-tagged JNK isoforms (p46JNK1alpha, p54JNK2alpha, p54JNK2beta,
      p46JNK3 and p54JNK3) were expressed in cultured rat PC12 phaeochromocytoma cells 
      and human small-cell lung cancer (SCLC) cells by retrovirus-mediated gene
      transfer. In addition, HA-tagged forms of the dual-specificity mitogen-activated 
      protein kinase kinases (MKKs), MKK4 and MKK7, which are specific activators of
      the JNK enzymes, were similarly expressed. Reverse transcription and PCR revealed
      that JNK3 is endogenously expressed in SCLC cells, but not in either chromaffin
      or neuronally differentiated PC12 cells. MKK4 and MKK7 were endogenously
      expressed in both PC12 cells and SHP77 cells. Immunoprecipitation and analysis of
      the JNKs expressed in SCLC cells revealed strong stimulation of all five JNK
      isoforms by UV radiation. Hypertonic stress, elicited by mannitol, also
      significantly stimulated these same JNKs, although the JNK3 isoforms were most
      strongly activated. In PC12 cell transfectants, however, selective and equal
      activation of p54JNK2alpha and p54JNK3 by UV and osmotic stress was observed,
      with little or no activation of JNK1alpha or JNK2beta. In contrast with the broad
      activation of the JNK enzymes by UV in SCLC cells, only HA-MKK4 was stimulated by
      UV exposure in these cells, whereas osmotic stress stimulated both HA-MKK4 and
      HA-MKK7. These findings indicate selective activation of JNK and MKK isoforms in 
      a manner that is dependent upon the specific cell stress and the cell type.
FAU - Butterfield, L
AU  - Butterfield L
AD  - Department of Medicine, University of Colorado Health Sciences Center, 4200 E.
      Ninth Ave, Denver, CO 80262, USA.
FAU - Zentrich, E
AU  - Zentrich E
FAU - Beekman, A
AU  - Beekman A
FAU - Heasley, L E
AU  - Heasley LE
LA  - eng
GR  - CA 46934/CA/NCI NIH HHS/United States
GR  - CA 58157/CA/NCI NIH HHS/United States
GR  - GM 48826/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA Primers)
RN  - 0 (Isoenzymes)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism
MH  - DNA Primers
MH  - Humans
MH  - Isoenzymes/genetics/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - *Mitogen-Activated Protein Kinases
MH  - Osmotic Pressure
MH  - PC12 Cells
MH  - Protein Kinases/genetics/*metabolism
MH  - Rats
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Ultraviolet Rays
PMC - PMC1220103
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Mar 15;338 ( Pt 3):681-6.