PMID- 10051191
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 88
IP  - 1
DP  - 1999 Jan
TI  - Increased expression of hippocampal cholinergic neurostimulating peptide-related 
      components and their messenger RNAs in the hippocampus of aged
      senescence-accelerated mice.
PG  - 79-92
AB  - Hippocampal cholinergic neurostimulating peptide stimulates cholinergic phenotype
      development by inducing choline acetyltransferase in the rat medial septal
      nucleus in vitro. Adult senescence-accelerated-prone mice/8, a substrain of the
      senescence-accelerated-prone mouse, show a remarkable age-accelerated
      deterioration in learning and memory. We cloned mouse hippocampal cholinergic
      neurostimulating peptide precursor protein complementary DNA. The deduced amino
      acid sequence showed that the neurostimulating peptide itself is the same as that
      found in the rat. In situ hybridization revealed that the highest expression of
      the precursor protein messenger RNA was in hippocampal pyramidal neurons.
      Compared with a strain of senescence-accelerated-resistant mouse (control mouse),
      adult senescence-accelerated-prone mice/8 showed increased expression of both the
      precursor messenger RNA and the neurostimulating peptide-related immunodeposits
      in the hippocampal CA1 field. The deposits were intensely and diffusely
      precipitated in neuropils throughout the strata oriens and radiatum in
      senescence-accelerated-prone mice/8, but not in control mice. The
      neurostimulating peptide content in the hippocampus was higher in
      senescence-accelerated-prone mice/8 than in control mice, while its precursor
      protein itself was not different between the two strains. Furthermore, our
      previous and present data show that the medial septal and hippocampal choline
      acetyltransferase activity was significantly lower in
      senescence-accelerated-prone mice/8 than in control mice. The data suggest that, 
      in hippocampal neurons in adult senescence-accelerated-prone mice/8, the
      production of hippocampal cholinergic neurostimulating peptide precursor protein 
      in neuronal somata, which is associated with an increased expression of its
      messenger RNA in the CA1 field, occurs as a consequence of low activity in their 
      presynaptic cholinergic neurons. This is followed by accelerated processing to
      generate bioactive peptide and transport to its functional fields. However,
      certain mechanisms reduce the release of the peptide and lead to its accumulation
      in the neuropil. These disturbances of the septohippocampal cholinergic system
      might be the biochemical mechanism underlying the characteristic deterioration of
      senescence-accelerated-prone mice/8.
FAU - Matsukawa, N
AU  - Matsukawa N
AD  - Second Department of Internal Medicine, Medical School, Nagoya City University,
      Nagoya, Japan.
FAU - Tooyama, I
AU  - Tooyama I
FAU - Kimura, H
AU  - Kimura H
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Tsugu, Y
AU  - Tsugu Y
FAU - Oomura, Y
AU  - Oomura Y
FAU - Ojika, K
AU  - Ojika K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Cholinergic Agents)
RN  - 0 (Neuropeptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 140158-49-2 (hippocampal cholinergic neurostimulating peptide)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Aging/genetics/*physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Choline O-Acetyltransferase/genetics
MH  - Cholinergic Agents
MH  - Cloning, Molecular
MH  - *Gene Expression Regulation
MH  - Hippocampus/cytology/*metabolism
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Neurons/cytology/*metabolism
MH  - Neuropeptides/biosynthesis/chemistry/*genetics
MH  - Pyramidal Cells/cytology/metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - *Transcription, Genetic
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0306452298002152 [pii]
AID - 10.1016/s0306-4522(98)00215-2 [doi]
PST - ppublish
SO  - Neuroscience. 1999 Jan;88(1):79-92. doi: 10.1016/s0306-4522(98)00215-2.