PMID- 10050885
OWN - NLM
STAT- MEDLINE
DCOM- 19990402
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 7
DP  - 1999 Feb 18
TI  - Search for oncogenic regulators in an autocrine tumor model using differential
      display PCR: identification of novel candidate genes including the calcium
      channel mtrp6.
PG  - 1487-94
AB  - A hemopoietic multistep tumor model, in which IL-3 dependent PB-3c mast cells,
      following expression of v-H-ras progress in vivo to IL-3 producing autocrine
      tumors has previously been established. Central for this oncogenic progression is
      a recessive step, which is reversible by cell fusion and leads to stabilization
      of IL-3 mRNA with concomitant activation of the autocrine loop. Comparing the
      IL-3 dependent PB-3c and the IL-3 autocrine V2D1 tumor cells with differential
      display PCR revealed 12 differentially expressed genes of which eight were
      upregulated and four downregulated in the tumor. They included four proteases
      (mouse mast cell protease 2, granzyme B, pepsinogen F and serine protease 1) and 
      two metabolic enzymes (adenine phosphoribosyltransferase and
      fructose1,6-bisphosphatase). For validation, expression of the identified genes
      was tested in independent PB-3c precursor clones and their tumor derivatives.
      Expression of an endogenous retroviral IAP element and three unknown transcripts 
      were consistently upregulated in all tumor lines. In somatic cell hybrids, two of
      these unknown cDNAs showed a dominant and one a recessive expression pattern. One
      transcript, expressed in the precursor but downregulated in the tumor cells, was 
      cloned and identified as the murine calcium channel mtrp6.
FAU - Buess, M
AU  - Buess M
AD  - Institute for Medical Microbiology, University of Basel, Switzerland.
FAU - Engler, O
AU  - Engler O
FAU - Hirsch, H H
AU  - Hirsch HH
FAU - Moroni, C
AU  - Moroni C
LA  - eng
SI  - GENBANK/AF057748
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Calcium Channels)
RN  - 0 (DNA, Complementary)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPC6 Cation Channel)
RN  - 0 (TRPC6 protein, human)
RN  - 0 (Trpc6 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium Channels/*genetics
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - DNA, Complementary
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mice
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - *Oncogenes
MH  - Polymerase Chain Reaction/*methods
MH  - TRPC Cation Channels
MH  - TRPC6 Cation Channel
MH  - Tumor Cells, Cultured
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - 10.1038/sj.onc.1202445 [doi]
PST - ppublish
SO  - Oncogene. 1999 Feb 18;18(7):1487-94. doi: 10.1038/sj.onc.1202445.