PMID- 10050851
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 397
IP  - 6720
DP  - 1999 Feb 18
TI  - A mammalian protein with specific demethylase activity for mCpG DNA.
PG  - 579-83
AB  - DNA-methylation patterns are important for regulating genome functions, and are
      determined by the enzymatic processes of methylation and demethylation. The
      demethylating enzyme has now been identified: a mammalian complementary DNA
      encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms
      methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA
      is translated or transiently transfected into human embryonal kidney cells in
      vitro. The discovery of this DNA demethylase should provide a basis for the
      molecular and developmental analysis of the role of DNA methylation and
      demethylation.
FAU - Bhattacharya, S K
AU  - Bhattacharya SK
AD  - Department of Pharmacology and Therapeutics, McGill University, Montreal, QC,
      Canada.
FAU - Ramchandani, S
AU  - Ramchandani S
FAU - Cervoni, N
AU  - Cervoni N
FAU - Szyf, M
AU  - Szyf M
LA  - eng
SI  - GENBANK/AF072242
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (DNA, Complementary)
RN  - 0 (Dinucleoside Phosphates)
RN  - 2382-65-2 (cytidylyl-3'-5'-guanosine)
RN  - 8J337D1HZY (Cytosine)
RN  - 9007-49-2 (DNA)
RN  - EC 1.- (Oxidoreductases, O-Demethylating)
RN  - EC 1.- (methyl-CpG DNA demethylase)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
CIN - Nature. 1999 Feb 18;397(6720):568-9. PMID: 10050847
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catalysis
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cytosine/metabolism
MH  - DNA/*metabolism
MH  - DNA Methylation
MH  - DNA, Complementary
MH  - Dinucleoside Phosphates/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Methanol/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oxidoreductases, O-Demethylating/genetics/*isolation & purification/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/03/02 03:05
MHDA- 2001/03/23 10:01
CRDT- 1999/03/02 03:05
PHST- 1999/03/02 03:05 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/03/02 03:05 [entrez]
AID - 10.1038/17533 [doi]
PST - ppublish
SO  - Nature. 1999 Feb 18;397(6720):579-83. doi: 10.1038/17533.