PMID- 10050036
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 3
DP  - 1999 Mar
TI  - Molecular shape and ATP binding activity of rat p50, a putative mammalian
      homologue of RuvB DNA helicase.
PG  - 487-94
AB  - Based on partial amino acid sequences of p50 purified from a high-salt buffer
      extract of a rat liver nuclear matrix fraction, p50 cDNA was cloned and
      sequenced, and its amino acid sequence was predicted. The sequence contained
      helicase motifs, and showed homology with RuvB DNA helicase of Thermus
      thermophilus and an open reading frame for an unknown 50.5 k protein of
      Saccharomyces cerevisiae. p50 was expressed as a GST-fusion protein and antiserum
      against the protein was generated. p50 was localized to the nuclear matrix by
      cell fractionation and immunoblotting. p50 bound to ATP-Sepharose beads.
      Ultracentrifugation and gel filtration analyses showed that p50 in rat liver and 
      Xenopus egg mitotic extracts exists as large complexes corresponding to 697 k and
      447 k, respectively. A 50 k protein reactive with p50 antibodies was detected not
      only in rat liver nuclei, but also in a Xenopus egg cytoplasm fraction and a S.
      cerevisiae extract. This suggests that this putative DNA helicase is present in a
      wide variety of species ranging from yeast to mammals.
FAU - Kikuchi, N
AU  - Kikuchi N
AD  - Department of Biochemistry, Faculty of Science, Research and Education Center for
      Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara,
      630-0101, Japan.
FAU - Gohshi, T
AU  - Gohshi T
FAU - Kawahire, S
AU  - Kawahire S
FAU - Tachibana, T
AU  - Tachibana T
FAU - Yoneda, Y
AU  - Yoneda Y
FAU - Isobe, T
AU  - Isobe T
FAU - Lim, C R
AU  - Lim CR
FAU - Kohno, K
AU  - Kohno K
FAU - Ichimura, T
AU  - Ichimura T
FAU - Omata, S
AU  - Omata S
FAU - Horigome, T
AU  - Horigome T
LA  - eng
SI  - GENBANK/AB001581
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Bacterial Proteins)
RN  - 0 (RuvB protein, Bacteria)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (Ruvbl1 protein, rat)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphate/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/*genetics/*metabolism
MH  - Base Sequence
MH  - DNA Helicases/*genetics/*metabolism
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Matrix/metabolism
MH  - Open Reading Frames/genetics
MH  - Rats
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Alignment
MH  - Substrate Specificity
MH  - Thermus thermophilus/genetics
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022312 [doi]
PST - ppublish
SO  - J Biochem. 1999 Mar;125(3):487-94. doi: 10.1093/oxfordjournals.jbchem.a022312.