PMID- 10050029
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 3
DP  - 1999 Mar
TI  - Molecular cloning of mouse and bovine chondromodulin-II cDNAs and the
      growth-promoting actions of bovine recombinant protein.
PG  - 436-42
AB  - We previously determined the complete primary sequence of a heparin-binding
      growth-promoting factor, chondromodulin-II (ChM-II), which stimulated the growth 
      of chondrocytes and osteoblasts in culture. Bovine ChM-II was a 16-kDa basic
      protein with 133 amino acid residues and exhibited a significant sequence
      similarity to the repeats of the chicken mim-1 gene product. Here we report the
      nucleotide sequences of bovine and mouse ChM-II cDNAs. The cDNAs each contained
      an open-reading frame corresponding to the ChM-II precursor with 151 amino acid
      residues. The N-terminus of the precursor included a secretory signal sequence of
      18 amino acids prior to the mature ChM-II sequence. Unlike MIM-1, there was no
      repeat structure in the precursor protein, indicating that ChM-II was encoded as 
      a gene product distinct from MIM-1. We then expressed recombinant bovine ChM-II
      protein which was purified to homogeneity. The recombinant protein stimulated the
      growth of rabbit growth plate chondrocytes, mouse MC3T3-E1 cells and rat UMR-106 
      osteoblastic cells in vitro.
FAU - Shukunami, C
AU  - Shukunami C
AD  - Department of Molecular Interaction and Tissue Engineering, Institute for
      Frontier Medical Sciences, Kyoto University, Kyoto, 606-8507, Japan.
FAU - Kondo, J
AU  - Kondo J
FAU - Wakai, H
AU  - Wakai H
FAU - Takahashi, K
AU  - Takahashi K
FAU - Inoue, H
AU  - Inoue H
FAU - Kamizono, A
AU  - Kamizono A
FAU - Hiraki, Y
AU  - Hiraki Y
LA  - eng
SI  - GENBANK/AF035161
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (DNA, Complementary)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (LECT2 protein, Bos taurus)
RN  - 0 (Lect2 protein, mouse)
RN  - 0 (Protein Precursors)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Chondrocytes/cytology/*drug effects
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Growth Substances/*genetics/pharmacology
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Osteoblasts/cytology/*drug effects
MH  - Protein Precursors/genetics
MH  - Proteins/*genetics/pharmacology
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/genetics/pharmacology
MH  - Sequence Alignment
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022305 [doi]
PST - ppublish
SO  - J Biochem. 1999 Mar;125(3):436-42. doi: 10.1093/oxfordjournals.jbchem.a022305.