PMID- 10049833
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20171116
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 255
IP  - 1
DP  - 1999 Mar 1
TI  - Interactions between Tat and TAR and human immunodeficiency virus replication are
      facilitated by human cyclin T1 but not cyclins T2a or T2b.
PG  - 182-9
AB  - The transcriptional transactivator (Tat) from the human immunodeficiency virus
      (HIV) does not function efficiently in Chinese hamster ovary (CHO) cells. Only
      somatic cell hybrids between CHO and human cells and CHO cells containing human
      chromosome 12 (CHO12) support high levels of Tat transactivation. This
      restriction was mapped to interactions between Tat and TAR. Recently, human
      cyclin T1 was found to increase the binding of Tat to TAR and levels of Tat
      transactivation in rodent cells. By combining individually with CDK9, cyclin T1
      or related cyclins T2a and T2b form distinct positive transcription elongation
      factor b (P-TEFb) complexes. In this report, we found that of these three
      cyclins, only cyclin T1 is encoded on human chromosome 12 and is responsible for 
      its effects in CHO cells. Moreover, only human cyclin T1, not mouse cyclin T1 or 
      human cyclins T2a or T2b, supported interactions between Tat and TAR in vitro.
      Finally, after introducing appropriate receptors and human cyclin T1 into CHO
      cells, they became permissive for infection by and replication of HIV.
CI  - Copyright 1999 Academic Press.
FAU - Wimmer, J
AU  - Wimmer J
AD  - Department of Medicine, University of California at San Francisco, San Francisco,
      California 94143-0703, USA.
FAU - Fujinaga, K
AU  - Fujinaga K
FAU - Taube, R
AU  - Taube R
FAU - Cujec, T P
AU  - Cujec TP
FAU - Zhu, Y
AU  - Zhu Y
FAU - Peng, J
AU  - Peng J
FAU - Price, D H
AU  - Price DH
FAU - Peterlin, B M
AU  - Peterlin BM
LA  - eng
GR  - AI13691/AI/NIAID NIH HHS/United States
GR  - GM 35500/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (CCNT1 protein, human)
RN  - 0 (CD4 Antigens)
RN  - 0 (Ccnt1 protein, mouse)
RN  - 0 (Cyclin T)
RN  - 0 (Cyclins)
RN  - 0 (Gene Products, tat)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Animals
MH  - CD4 Antigens/genetics/metabolism
MH  - CHO Cells
MH  - Cell Line, Transformed
MH  - Chromosomes, Human, Pair 12
MH  - Cricetinae
MH  - Cyclin T
MH  - Cyclins/genetics/*metabolism
MH  - Gene Expression Regulation, Viral
MH  - Gene Products, tat/genetics/*metabolism
MH  - *HIV Long Terminal Repeat
MH  - HIV-1/genetics/*physiology
MH  - HeLa Cells
MH  - Humans
MH  - Jurkat Cells
MH  - Mice
MH  - Proviruses/genetics
MH  - Receptors, CCR5/genetics/metabolism
MH  - Receptors, CXCR4/genetics/metabolism
MH  - Receptors, Chemokine
MH  - Transcriptional Activation
MH  - *Virus Replication
MH  - tat Gene Products, Human Immunodeficiency Virus
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - 10.1006/viro.1998.9589 [doi]
AID - S0042-6822(98)99589-7 [pii]
PST - ppublish
SO  - Virology. 1999 Mar 1;255(1):182-9. doi: 10.1006/viro.1998.9589.