PMID- 10049781
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 3
DP  - 1999 Feb 24
TI  - Hepatocyte growth factor activator inhibitor type 2 lacking the first Kunitz-type
      serine proteinase inhibitor domain is a predominant product in mouse but not in
      human.
PG  - 740-8
AB  - Hepatocyte growth factor activator inhibitor type 2 (HAI-2) is a new Kunitz-type 
      serine protease inhibitor, which is purified and cloned from human stomach cancer
      cell line MKN45. The mature HAI-2 protein contains two Kunitz domains and the
      first domain is mainly responsible for the inhibitory activity against hepatocyte
      growth factor activator (HGFA). In this study, we identified the mouse homolog of
      HAI-2 (mHAI-2) by screening the data base of public expressed sequence tag
      (dbEST). In addition to a full-length cDNA corresponding to human HAI-2, a
      shorter size of mHAI-2 cDNA was obtained from mouse kidney by
      reverse-transcription polymerase chain reaction (RT-PCR). Sequence analysis of
      this shorter cDNA revealed that the region encoding the first Kunitz domain was
      completely deleted. Analysis of mouse genomic DNA showed that the deleted cDNA
      was generated by an alternative splicing mechanism. Surprisingly, the spliced
      form lacking the first Kunitz domain was a predominant transcript in all tissues 
      of mice tested but not in those of human as assessed by RT-PCR analysis. This
      phenomenon is also confirmed by Western blot analysis using the specific
      antiserum against human HAI-2 protein. These results suggest that most of HAI-2
      expressed in various tissues of mice may be unable to inhibit HGFA efficiently.
CI  - Copyright 1999 Academic Press.
FAU - Itoh, H
AU  - Itoh H
AD  - Second Department of Pathology, Miyazaki Medical College, Kihara, Miyazaki,
      Kiyotake, 889-1692, Japan.
FAU - Kataoka, H
AU  - Kataoka H
FAU - Hamasuna, R
AU  - Hamasuna R
FAU - Kitamura, N
AU  - Kitamura N
FAU - Koono, M
AU  - Koono M
LA  - eng
SI  - GENBANK/AF099016
SI  - GENBANK/AF099019
SI  - GENBANK/AF099020
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SPINT2 protein, human)
RN  - 9088-41-9 (Trypsin Inhibitor, Kunitz Soybean)
RN  - EC 3.4.21.- (HGF activator)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Expressed Sequence Tags
MH  - Humans
MH  - Introns/genetics
MH  - Membrane Glycoproteins/chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Placenta/metabolism
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion/genetics
MH  - Serine Endopeptidases/metabolism
MH  - *Trypsin Inhibitor, Kunitz Soybean
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90268-1 [pii]
AID - 10.1006/bbrc.1999.0268 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 24;255(3):740-8. doi:
      10.1006/bbrc.1999.0268.