PMID- 10049780
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 3
DP  - 1999 Feb 24
TI  - Antiangiogenic activity of restin, NC10 domain of human collagen XV: comparison
      to endostatin.
PG  - 735-9
AB  - Based on a homology search with endostatin, the C-terminus 185 aa of collagen
      XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa
      human collagen XV fragment, that we have named restin. Restin was expressed in
      the prokaryotic pET expression system. We have shown that restin inhibits the
      migration of endothelial cells in vitro but has no effect on the proliferation of
      these cells. A polyclonal antibody raised against endostatin cross-reacted with
      restin. Systemic administration of restin suppressed the growth of tumors in a
      xenograft renal carcinoma model.
CI  - Copyright 1999 Academic Press.
FAU - Ramchandran, R
AU  - Ramchandran R
AD  - Renal Division, Department of Medicine, Beth Israel Deaconess Medical Center and 
      Harvard Medical School, Boston, Massachusetts 02215, USA.
FAU - Dhanabal, M
AU  - Dhanabal M
FAU - Volk, R
AU  - Volk R
FAU - Waterman, M J
AU  - Waterman MJ
FAU - Segal, M
AU  - Segal M
FAU - Lu, H
AU  - Lu H
FAU - Knebelmann, B
AU  - Knebelmann B
FAU - Sukhatme, V P
AU  - Sukhatme VP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Collagen Type XVIII)
RN  - 0 (Endostatins)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 148349-95-5 (cytoplasmic linker protein 170)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antineoplastic Agents/pharmacology
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cloning, Molecular
MH  - Collagen/*chemistry/*pharmacology
MH  - Collagen Type XVIII
MH  - Endostatins
MH  - Humans
MH  - Intermediate Filament Proteins/*genetics/pharmacology
MH  - *Microtubule-Associated Proteins
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/pharmacology
MH  - Neoplasms, Experimental/metabolism
MH  - Neovascularization, Physiologic/*drug effects
MH  - Peptide Fragments/*genetics/*pharmacology
MH  - Recombinant Proteins/genetics/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Transplantation, Heterologous/immunology
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90248-6 [pii]
AID - 10.1006/bbrc.1999.0248 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 24;255(3):735-9. doi:
      10.1006/bbrc.1999.0248.