PMID- 10049752
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 3
DP  - 1999 Feb 24
TI  - Molecular cloning and functional characterization of rat delta-6 fatty acid
      desaturase.
PG  - 575-9
AB  - Mammalian cDNA fragments putatively encoding amino acid sequences characteristic 
      of the fatty acid desaturase were obtained using expressed sequence tag (EST)
      sequence informations. These fragments were subsequently used to screen a rat
      liver cDNA library, yielding a 1573-bp clone. Expression of DNA fragment
      containing either of two possible open reading frames (nucleotide numbers 97-1431
      and 148-1431) of the isolated clone in yeast led to the accumulation of
      gamma-linolenic acid in the presence of exogenous linoleic acid. In this system, 
      the addition of alpha-linolenic acid also resulted in the accumulation of its
      Delta-6 desaturated product whereas dihomo-gamma-linolenic acid failed to be a
      substrate. These results indicate that the protein encoded by the rat cDNA is
      Delta-6 fatty acid desaturase, and the first 17 amino acids corresponding to the 
      coding region 97-147 of the clone are not required to function in yeast.
CI  - Copyright 1999 Academic Press.
FAU - Aki, T
AU  - Aki T
AD  - Department of Molecular Biotechnology, Hiroshima University, 1-4-1 Kagamiyama,
      Higashi-Hiroshima, 739-8527, Japan. aki@ipc.hiroshima-ac.jp
FAU - Shimada, Y
AU  - Shimada Y
FAU - Inagaki, K
AU  - Inagaki K
FAU - Higashimoto, H
AU  - Higashimoto H
FAU - Kawamoto, S
AU  - Kawamoto S
FAU - Shigeta, S
AU  - Shigeta S
FAU - Ono, K
AU  - Ono K
FAU - Suzuki, O
AU  - Suzuki O
LA  - eng
SI  - GENBANK/AB021980
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Fatty Acids)
RN  - 0 (Linolenic Acids)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - EC 1.14.19.- (Fatty Acid Desaturases)
RN  - EC 1.14.19.3 (Linoleoyl-CoA Desaturase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromatography, Gas
MH  - Cloning, Molecular
MH  - Fatty Acid Desaturases/*genetics/metabolism
MH  - Fatty Acids/analysis
MH  - Linoleic Acid/metabolism
MH  - Linolenic Acids/metabolism
MH  - Linoleoyl-CoA Desaturase
MH  - Liver/*enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microsatellite Repeats/genetics
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90235-8 [pii]
AID - 10.1006/bbrc.1999.0235 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 24;255(3):575-9. doi:
      10.1006/bbrc.1999.0235.