PMID- 10049742
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20040617
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 2
DP  - 1999 Feb 16
TI  - Analysis of the consensus binding sequence and the DNA-binding domain of ZF5.
PG  - 528-34
AB  - Murine ZF5 is a transcription factor with five zinc finger motifs that represses 
      the c-myc gene by binding to two GC-rich elements at the promoter region. Because
      of its ubiquitous expression in a variety of tissues, elucidation of biological
      functions and cellular target genes of ZF5 is of great interest. As the first
      step of identifying cellular target genes, we have attempted to determine the
      consensus binding motif for ZF5. We succeeded in isolating 19 oligonucleotide
      duplex DNAs to which ZF5 binds and determined the binding sequences with DNase I 
      footprinting analysis. From these sequences, we deduced the consensus binding
      motif for ZF5 to be GSGCGCGR. In addition, we have analyzed the DNA-binding
      domain of ZF5 by testing a series of deletion mutants. It turned out that the
      zinc fingers 3 and 4 of the five finger motifs play a critical role in DNA
      binding.
CI  - Copyright 1999 Academic Press.
FAU - Obata, T
AU  - Obata T
AD  - Department of Dermatology, Hiroshima University School of Medicine, Japan.
      tobata@ipc.hiroshima-u.ac.jp
FAU - Yanagidani, A
AU  - Yanagidani A
FAU - Yokoro, K
AU  - Yokoro K
FAU - Numoto, M
AU  - Numoto M
FAU - Yamamoto, S
AU  - Yamamoto S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 0 (Oligonucleotides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Repressor Proteins)
RN  - 0 (Zfp161 protein, mouse)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - *Consensus Sequence
MH  - DNA/*chemistry/metabolism
MH  - DNA-Binding Proteins/*chemistry/metabolism
MH  - Mice
MH  - Nucleic Acid Heteroduplexes/chemistry/metabolism
MH  - Oligonucleotides/chemistry/metabolism
MH  - Peptide Fragments/*chemistry/metabolism
MH  - Repressor Proteins/*chemistry/metabolism
MH  - *Zinc Fingers
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(98)99970-3 [pii]
AID - 10.1006/bbrc.1998.9970 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 16;255(2):528-34. doi:
      10.1006/bbrc.1998.9970.