PMID- 10049739
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 2
DP  - 1999 Feb 16
TI  - Molecular cloning and characterization of two novel human renal organic anion
      transporters (hOAT1 and hOAT3).
PG  - 508-14
AB  - The cloned organic anion transporters from rat, mouse, and winter flounder
      (rOAT1, mOAT1, fROAT) mediate the coupled exchange of alpha-ketoglutarate with
      multiple organic anions, including p-aminohippurate (PAH). We have isolated two
      novel gene products from human kidney which bear significant homology to the
      known OATs and belong to the amphiphilic solute facilitator (ASF) family. The
      cDNAs, hOAT1 and hOAT3, encode for 550- and 568-amino-acid residue proteins,
      respectively. hOAT1 and hOAT3 mRNAs are expressed strongly in kidney and weakly
      in brain. Both genes map to chromosome 11 region q11.7. PAH uptake by Xenopus
      laevis oocytes injected with hOAT1 mRNA is increased 100-fold compared to
      water-injected oocytes. PAH uptake is chloride dependent and is not further
      increased by preincubation of oocytes in 5 mM glutarate. Uptake of PAH is
      inhibited by probenicid, alpha-ketoglutarate, bumetanide, furosemide, and
      losartan, but not by salicylate, urate, choline, amilioride, and
      hydrochlorothiazide.
CI  - Copyright 1999 Academic Press.
FAU - Race, J E
AU  - Race JE
AD  - Department of Medicine, State University of New York Health Science Center,
      Syracuse 13210, USA.
FAU - Grassl, S M
AU  - Grassl SM
FAU - Williams, W J
AU  - Williams WJ
FAU - Holtzman, E J
AU  - Holtzman EJ
LA  - eng
SI  - GENBANK/AF097490
SI  - GENBANK/AF097491
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Organic Anion Transporters, Sodium-Independent)
RN  - 0 (organic anion transport protein 3)
RN  - Y79XT83BJ9 (p-Aminohippuric Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anion Transport Proteins
MH  - Biological Transport
MH  - Blotting, Northern
MH  - Carrier Proteins/*chemistry/*genetics/physiology
MH  - Chromosomes, Human, Pair 11
MH  - Cloning, Molecular
MH  - Humans
MH  - Kidney/*chemistry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oocytes/metabolism
MH  - *Organic Anion Transporters, Sodium-Independent
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Xenopus laevis
MH  - p-Aminohippuric Acid/metabolism
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(98)99978-8 [pii]
AID - 10.1006/bbrc.1998.9978 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 16;255(2):508-14. doi:
      10.1006/bbrc.1998.9978.