PMID- 10049729 OWN - NLM STAT- MEDLINE DCOM- 19990311 LR - 20191210 IS - 0006-291X (Print) IS - 0006-291X (Linking) VI - 255 IP - 2 DP - 1999 Feb 16 TI - N-linked oligosaccharides of vomeromodulin, a putative pheromone transporter in rat. PG - 451-5 AB - Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages. CI - Copyright 1999 Academic Press. FAU - Mechref, Y AU - Mechref Y AD - Department of Chemistry, Indiana University, Bloomington 47405, USA. FAU - Ma, W AU - Ma W FAU - Hao, G AU - Hao G FAU - Novotny, M V AU - Novotny MV LA - eng GR - DC 02418/DC/NIDCD NIH HHS/United States GR - GM24349/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Carrier Proteins) RN - 0 (Glycoproteins) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (Membrane Transport Proteins) RN - 0 (Oligosaccharides) RN - 0 (Pheromones) RN - 0 (Polysaccharides) RN - 0 (Sialic Acids) RN - 0 (vomeromodulin protein, rat) RN - EC 3.2.1.- (Glycoside Hydrolases) SB - IM MH - Animals MH - Biological Transport MH - Carbohydrate Conformation MH - Carrier Proteins/*chemistry/metabolism MH - Chromatography, Affinity MH - Electrophoresis, Polyacrylamide Gel MH - Female MH - Glycoproteins/*chemistry/metabolism MH - Glycoside Hydrolases/metabolism MH - Glycosylation MH - Intercellular Signaling Peptides and Proteins MH - Male MH - *Membrane Transport Proteins MH - Oligosaccharides/*chemistry/metabolism MH - Pheromones/*chemistry/metabolism MH - Polysaccharides/chemistry/metabolism MH - Rats MH - Sialic Acids/chemistry MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization EDAT- 1999/03/02 00:00 MHDA- 1999/03/02 00:01 CRDT- 1999/03/02 00:00 PHST- 1999/03/02 00:00 [pubmed] PHST- 1999/03/02 00:01 [medline] PHST- 1999/03/02 00:00 [entrez] AID - S0006-291X(99)90176-6 [pii] AID - 10.1006/bbrc.1999.0176 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 1999 Feb 16;255(2):451-5. doi: 10.1006/bbrc.1999.0176.