PMID- 10049703
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20220409
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 2
DP  - 1999 Feb 16
TI  - Altered expression of hepatic CYP2E1 and CYP4A in obese, diabetic ob/ob mice, and
      fa/fa Zucker rats.
PG  - 300-6
AB  - Hepatic levels of the cytochrome P450 (CYP) proteins 2E1 and 4A are often
      increased in obesity, diabetes and fasting. In such states of nutritional
      imbalance, CYPs 2E1 and 4A may play a more significant role in fatty acid
      oxidation. In order to more fully characterize the regulation of CYP2E1 and CYP4A
      in obesity and obesity-related (type II) diabetes, we analyzed the hepatic
      expression of CYP2E1 and CYP4A in ob/ob mice which are leptin deficient, and
      fa/fa Zucker rats which have defective leptin receptor function. CYP2E1 protein
      and mRNA were either unchanged or reduced in both models. Conversely, expression 
      of murine Cyp4a10 and 4a14 in the obese mice, and 4A2 in the male fatty Zucker
      rat, were greatly increased. The levels of other CYP4As were either unchanged or 
      reduced. These results show that CYP2E1 is not inevitably increased by obesity
      and diabetes and indicate differential regulation of CYP4A subfamily genes in
      rodent models. Further, they implicate leptin receptor signaling as a factor that
      may modulate expression of CYP gene products involved in fatty acid oxidation.
CI  - Copyright 1999 Academic Press.
FAU - Enriquez, A
AU  - Enriquez A
AD  - Department of Medicine, University of Sydney, Westmead Hospital, New South Wales,
      Australia.
FAU - Leclercq, I
AU  - Leclercq I
FAU - Farrell, G C
AU  - Farrell GC
FAU - Robertson, G
AU  - Robertson G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EC 1.14.15.3 (Cytochrome P-450 CYP4A)
RN  - H0DE420U8G (Chlorzoxazone)
SB  - IM
MH  - Animals
MH  - Chlorzoxazone/metabolism
MH  - Cytochrome P-450 CYP2E1/*biosynthesis/genetics
MH  - Cytochrome P-450 CYP4A
MH  - Cytochrome P-450 Enzyme System/*biosynthesis/genetics
MH  - Diabetes Mellitus/*enzymology/genetics/physiopathology
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Microsomes, Liver/*enzymology
MH  - Mixed Function Oxygenases/*biosynthesis/genetics
MH  - *Obesity
MH  - Rats
MH  - Rats, Zucker
MH  - Sex Factors
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90202-4 [pii]
AID - 10.1006/bbrc.1999.0202 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 16;255(2):300-6. doi:
      10.1006/bbrc.1999.0202.