PMID- 10049700
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 2
DP  - 1999 Feb 16
TI  - Human LAT1, a subunit of system L amino acid transporter: molecular cloning and
      transport function.
PG  - 283-8
AB  - We report here on the cloning and functional characterization of human LAT1, a
      subunit of the amino acid transport system L. The hLAT1 cDNA, obtained from a
      human placental cDNA library, codes for a protein of 507 amino acids. When
      functionally expressed in mammalian cells together with the heavy chain of the
      rat 4F2 antigen (r4F2hc), hLAT1 induces the transport of neutral amino acids.
      When expressed independently, neither hLAT1 nor r4F2hc was capable of amino acid 
      transport to any significant extent. Thus, the hLAT1-r4F2hc heterodimeric complex
      is responsible for the observed amino acid transport. The transport process
      induced by the heterodimer is Na+ independent and is not influenced by pH. It
      recognizes exclusively neutral amino acids with high affinity. LAT1-specific mRNA
      is expressed in most human tissues with the notable exception of the intestine.
CI  - Copyright 1999 Academic Press.
FAU - Prasad, P D
AU  - Prasad PD
AD  - Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta
      30912, USA.
FAU - Wang, H
AU  - Wang H
FAU - Huang, W
AU  - Huang W
FAU - Kekuda, R
AU  - Kekuda R
FAU - Rajan, D P
AU  - Rajan DP
FAU - Leibach, F H
AU  - Leibach FH
FAU - Ganapathy, V
AU  - Ganapathy V
LA  - eng
SI  - GENBANK/AF104032
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amino Acid Transport Systems)
RN  - 0 (Antigens, CD)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fusion Regulatory Protein-1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Transport Systems
MH  - Animals
MH  - Antigens, CD/biosynthesis/physiology
MH  - Biological Transport
MH  - Blotting, Northern
MH  - Carrier Proteins/biosynthesis/chemistry/*genetics/*physiology
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Fusion Regulatory Protein-1
MH  - Gene Library
MH  - Humans
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Placenta/chemistry
MH  - Rats
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90206-1 [pii]
AID - 10.1006/bbrc.1999.0206 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 16;255(2):283-8. doi:
      10.1006/bbrc.1999.0206.