PMID- 10049565
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20071114
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 207
IP  - 1
DP  - 1999 Mar 1
TI  - Cloning and functional studies of a novel gene aberrantly expressed in
      RB-deficient embryos.
PG  - 62-75
AB  - The tumor suppressor RB regulates diverse cellular processes such as G1/S
      transition, cell differentiation, and cell survival. Indeed, Rb-knockout mice
      exhibit phenotypes including ectopic mitosis, defective differentiation, and
      extensive apoptosis in the neurons. Using differential display, a novel gene,
      Rig-1, was isolated based on its elevated expression in the hindbrain and spinal 
      cord of Rb-knockout embryos. The longest open reading frame of Rig-1 encoded a
      polypeptide that consists of a putative extracellular segment with five
      immunoglobulin-like domains and three fibronectin III-like domains, a putative
      transmembrane domain, and a distinct intracellular segment. The Rig-1 sequence
      was 40% identical to the recently identified roundabout protein. Consistent with 
      the predicted transmembrane nature of the protein, Rig-1 protein was present in
      the membranous fraction. Antisera raised against the putative extracellular and
      intracellular segments of Rig-1 reacted with an approximately 210-kDa protein in 
      mouse embryonic CNS. Rig-1 mRNA was transiently expressed in the embryonic
      hindbrain and spinal cord. Elevated levels of Rig-1 mRNA and protein were found
      in Rb-/- embryos. Ectopic expression of a transmembrane form of Rig-1, but not
      the secreted form, promoted neuronal cell entrance to S phase and repressed the
      expression of a marker of differentiated neuron, Talpha1 tubulin. Thus Rig-1, a
      possible distant relative of roundabout, may mediate some of the pleiotropic
      roles of RB in the developing neurons.
CI  - Copyright 1999 Academic Press.
FAU - Yuan, S S
AU  - Yuan SS
AD  - Department of Molecular Medicine/Institute of Biotechnology, University of Texas 
      Health Science Center at San Antonio, San Antonio, Texas, 78245, USA.
FAU - Cox, L A
AU  - Cox LA
FAU - Dasika, G K
AU  - Dasika GK
FAU - Lee, E Y
AU  - Lee EY
LA  - eng
SI  - GENBANK/AF060570
GR  - CA49649/CA/NCI NIH HHS/United States
GR  - HD30265/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Robo3 protein, mouse)
RN  - 0 (roundabout protein)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle/genetics
MH  - Central Nervous System/embryology/growth & development
MH  - Cloning, Molecular
MH  - *Embryonic and Fetal Development
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Immunoglobulins/genetics
MH  - In Situ Hybridization
MH  - Membrane Proteins/chemistry/*genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*genetics
MH  - RNA, Messenger/genetics
MH  - Receptors, Immunologic/genetics
MH  - Retinoblastoma Protein/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0012-1606(98)99141-2 [pii]
AID - 10.1006/dbio.1998.9141 [doi]
PST - ppublish
SO  - Dev Biol. 1999 Mar 1;207(1):62-75. doi: 10.1006/dbio.1998.9141.