PMID- 10049363
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20191210
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 4
DP  - 1999 Feb 15
TI  - Role of the Bicoid-related homeodomain factor Pitx1 in specifying hindlimb
      morphogenesis and pituitary development.
PG  - 484-94
AB  - Pitx1 is a Bicoid-related homeodomain factor that exhibits preferential
      expression in the hindlimb, as well as expression in the developing anterior
      pituitary gland and first branchial arch. Here, we report that Pitx1 gene-deleted
      mice exhibit striking abnormalities in morphogenesis and growth of the hindlimb, 
      resulting in a limb that exhibits structural changes in tibia and fibula as well 
      as patterning alterations in patella and proximal tarsus, to more closely
      resemble the corresponding forelimb structures. Deletion of the Pitx1 locus
      results in decreased distal expression of the hindlimb-specific marker, the T-box
      factor, Tbx4. On the basis of similar expression patterns in chick, targeted
      misexpression of chick Pitx1 in the developing wing bud causes the resulting limb
      to assume altered digit number and morphogenesis, with Tbx4 induction. We
      hypothesize that Pitx1 serves to critically modulate morphogenesis, growth, and
      potential patterning of a specific hindlimb region, serving as a component of the
      morphological and growth distinctions in forelimb and hindlimb identity. Pitx1
      gene-deleted mice also exhibit reciprocal abnormalities of two ventral and one
      dorsal anterior pituitary cell types, presumably on the basis of its synergistic 
      functions with other transcription factors, and defects in the derivatives of the
      first branchial arch, including cleft palate, suggesting a proliferative defect
      in these organs analogous to that observed in the hindlimb.
FAU - Szeto, D P
AU  - Szeto DP
AD  - Howard Hughes Medical Institute, University of California, San Diego, School and 
      Department of Medicine, La Jolla, California 92093-0648, USA.
FAU - Rodriguez-Esteban, C
AU  - Rodriguez-Esteban C
FAU - Ryan, A K
AU  - Ryan AK
FAU - O'Connell, S M
AU  - O'Connell SM
FAU - Liu, F
AU  - Liu F
FAU - Kioussi, C
AU  - Kioussi C
FAU - Gleiberman, A S
AU  - Gleiberman AS
FAU - Izpisua-Belmonte, J C
AU  - Izpisua-Belmonte JC
FAU - Rosenfeld, M G
AU  - Rosenfeld MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Avian Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (TBX4 protein, Gallus gallus)
RN  - 0 (Tbx4 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (bcd protein, Drosophila)
RN  - 0 (homeobox protein PITX1)
RN  - 0 (homeobox protein PITX3)
RN  - 184787-43-7 (homeobox protein PITX2)
SB  - IM
MH  - Animals
MH  - *Avian Proteins
MH  - Branchial Region/embryology/growth & development
MH  - Chick Embryo
MH  - Drosophila Proteins
MH  - Gene Deletion
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Hindlimb/embryology/*growth & development
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Mandible/embryology/growth & development
MH  - Mice
MH  - Mice, Knockout
MH  - Morphogenesis/*genetics
MH  - *Nuclear Proteins
MH  - Paired Box Transcription Factors
MH  - Pituitary Gland/embryology/*growth & development
MH  - *T-Box Domain Proteins
MH  - Trans-Activators/*genetics/metabolism
MH  - Transcription Factors/*genetics
MH  - Wings, Animal/embryology/*growth & development
PMC - PMC316471
EDAT- 1999/02/27 00:00
MHDA- 1999/02/27 00:01
CRDT- 1999/02/27 00:00
PHST- 1999/02/27 00:00 [pubmed]
PHST- 1999/02/27 00:01 [medline]
PHST- 1999/02/27 00:00 [entrez]
AID - 10.1101/gad.13.4.484 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Feb 15;13(4):484-94. doi: 10.1101/gad.13.4.484.