PMID- 10048485
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190513
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 5
IP  - 6
DP  - 1998 Dec 31
TI  - Prediction of the coding sequences of unidentified human genes. XII. The complete
      sequences of 100 new cDNA clones from brain which code for large proteins in
      vitro.
PG  - 355-64
AB  - In this paper, we report the sequences of 100 cDNA clones newly determined from a
      set of size-fractionated human brain cDNA libraries and predict the coding
      sequences of the corresponding genes, named KIAA0819 to KIAA0918. These cDNA
      clones were selected on the basis of their coding potentials of large proteins
      (50 kDa and more) by using in vitro transcription/translation assays. The
      sequence data showed that the average sizes of the inserts and corresponding open
      reading frames are 4.4 kb and 2.5 kb (831 amino acid residues), respectively.
      Homology and motif/domain searches against the public databases indicated that
      the predicted coding sequences of 83 genes were similar to those of known genes, 
      59% of which (49 genes) were categorized as coding for proteins functionally
      related to cell signaling/communication, cell structure/motility and nucleic acid
      management. The chromosomal locations and the expression profiles of all the
      genes were also examined. For 54 clones including brain-specific ones, the mRNA
      levels were further examined among 8 brain regions (amygdala, corpus callosum,
      cerebellum, caudate nucleus, hippocampus, substantia nigra, subthalamic nucleus, 
      and thalamus), spinal cord, and fetal brain.
FAU - Nagase, T
AU  - Nagase T
AD  - Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
FAU - Ishikawa, K
AU  - Ishikawa K
FAU - Suyama, M
AU  - Suyama M
FAU - Kikuno, R
AU  - Kikuno R
FAU - Hirosawa, M
AU  - Hirosawa M
FAU - Miyajima, N
AU  - Miyajima N
FAU - Tanaka, A
AU  - Tanaka A
FAU - Kotani, H
AU  - Kotani H
FAU - Nomura, N
AU  - Nomura N
FAU - Ohara, O
AU  - Ohara O
LA  - eng
SI  - GENBANK/AB020626
SI  - GENBANK/AB020627
SI  - GENBANK/AB020628
SI  - GENBANK/AB020629
SI  - GENBANK/AB020630
SI  - GENBANK/AB020631
SI  - GENBANK/AB020632
SI  - GENBANK/AB020633
SI  - GENBANK/AB020634
SI  - GENBANK/AB020635
SI  - GENBANK/AB020636
SI  - GENBANK/AB020637
SI  - GENBANK/AB020638
SI  - GENBANK/AB020639
SI  - GENBANK/AB020640
SI  - GENBANK/AB020641
SI  - GENBANK/AB020642
SI  - GENBANK/AB020643
SI  - GENBANK/AB020644
SI  - GENBANK/AB020645
SI  - GENBANK/AB020646
SI  - GENBANK/AB020647
SI  - GENBANK/AB020648
SI  - GENBANK/AB020649
SI  - GENBANK/AB020650
SI  - GENBANK/AB020651
SI  - GENBANK/AB020652
SI  - GENBANK/AB020653
SI  - GENBANK/AB020654
SI  - GENBANK/AB020655
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes
      and genomes
JID - 9423827
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Amino Acid Sequence
MH  - Brain Chemistry/*genetics
MH  - Chromosome Mapping
MH  - *DNA, Complementary
MH  - Databases, Factual
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - *Gene Library
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/02/27 00:00
MHDA- 1999/02/27 00:01
CRDT- 1999/02/27 00:00
PHST- 1999/02/27 00:00 [pubmed]
PHST- 1999/02/27 00:01 [medline]
PHST- 1999/02/27 00:00 [entrez]
AID - 10.1093/dnares/5.6.355 [doi]
PST - ppublish
SO  - DNA Res. 1998 Dec 31;5(6):355-64. doi: 10.1093/dnares/5.6.355.