PMID- 10037746
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 10
DP  - 1999 Mar 5
TI  - Molecular characterization of a third member of the guanylyl cyclase-activating
      protein subfamily.
PG  - 6526-35
AB  - The mammalian retina contains at least two guanylyl cyclases (GC1 and GC2) and
      two guanylyl cyclase-activating proteins (GCAP1 and GCAP2). Here we present
      evidence of the presence of a new photoreceptor-specific GCAP, termed GCAP3,
      which is closely related to GCAP1. The sequence similarity of GCAP3 with GCAP1
      and GCAP2 is 57 and 49%, respectively. Recombinant GCAP3 and GCAP2 stimulate GC1 
      and GC2 in low [Ca2+]free and inhibit GCs when [Ca2+]free is elevated, unlike
      GCAP1, which only stimulates GC1. GCAP3 is encoded by a distinct gene present in 
      other mammalian species but could not be detected by genomic Southern blotting in
      rodents, amphibians, and lower vertebrates. The intron/exon arrangement of the
      GCAP3 gene is identical to that of the other GCAP genes. While the GCAP1 and
      GCAP2 genes are arranged in a tail-to-tail array on chromosome 6p in human, the
      GCAP3 gene is located on 3q13.1, suggesting an ancestral gene
      duplication/translocation event. The identification of multiple Ca2+-binding
      proteins that interact with GC is suggestive of complex regulatory mechanisms for
      photoreceptor GC.
FAU - Haeseleer, F
AU  - Haeseleer F
AD  - Department of Ophthalmology, University of Washington School of Medicine,
      Seattle, Washington 98195, USA.
FAU - Sokal, I
AU  - Sokal I
FAU - Li, N
AU  - Li N
FAU - Pettenati, M
AU  - Pettenati M
FAU - Rao, N
AU  - Rao N
FAU - Bronson, D
AU  - Bronson D
FAU - Wechter, R
AU  - Wechter R
FAU - Baehr, W
AU  - Baehr W
FAU - Palczewski, K
AU  - Palczewski K
LA  - eng
SI  - GENBANK/AF109998
SI  - GENBANK/AF109999
SI  - GENBANK/AF110000
SI  - GENBANK/AF110001
SI  - GENBANK/AF110002
SI  - GENBANK/AF110003
GR  - EY08061/EY/NEI NIH HHS/United States
GR  - EY08123/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (GUCA1A protein, human)
RN  - 0 (GUCA1B protein, human)
RN  - 0 (GUCA1C protein, human)
RN  - 0 (Guanylate Cyclase-Activating Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium-Binding Proteins/*genetics/metabolism
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Guanylate Cyclase/*metabolism
MH  - Guanylate Cyclase-Activating Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics
MH  - Photoreceptor Cells/*metabolism
MH  - Retina/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis
MH  - Species Specificity
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - 10.1074/jbc.274.10.6526 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 5;274(10):6526-35. doi: 10.1074/jbc.274.10.6526.