PMID- 10037722
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 10
DP  - 1999 Mar 5
TI  - Cloning of a stretch-inhibitable nonselective cation channel.
PG  - 6330-5
AB  - A homologue of the capsaicin receptor-nonselective cation channel was cloned from
      the rat kidney to investigate a mechanosensitive channel. We found this channel
      to be inactivated by membrane stretch and have designated it stretch-inactivated 
      channel (SIC). SIC encodes a 563-amino acid protein with putative six
      transmembrane segments. The cDNA was expressed in mammalian cells, and
      electophysiological studies were performed. SIC-induced large cation currents
      were found to be regulated by cell volume, with currents being stimulated by cell
      shrinkage and inhibited by cell swelling. Single channel analysis showed a
      conductance of 250 pS with cation permeability (PCl/PNa < 0.1), and the channel
      possessed some of the characteristics of a stretch-inactivated channel in that it
      was permeable to calcium, sensitive to membrane stretch, and blocked by Gd3+.
      Therefore, we cloned one of the mechanosensitive cation channels of mammals,
      which is considered to regulate Ca2+ influx in response to mechanical stress on
      the cell membrane.
FAU - Suzuki, M
AU  - Suzuki M
AD  - Department of Pharmacology, Jichi Medical School, Minamikawachi, Tochigi 329-04, 
      Japan. macsuz@jichi.ac.jp
FAU - Sato, J
AU  - Sato J
FAU - Kutsuwada, K
AU  - Kutsuwada K
FAU - Ooki, G
AU  - Ooki G
FAU - Imai, M
AU  - Imai M
LA  - eng
SI  - GENBANK/AB015231
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Ion Channels)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Drug)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/genetics
MH  - *Ion Channel Gating
MH  - Ion Channels/*genetics
MH  - Kidney/*metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Drug/*genetics
MH  - Stress, Mechanical
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - 10.1074/jbc.274.10.6330 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 5;274(10):6330-5. doi: 10.1074/jbc.274.10.6330.