PMID- 10037142
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 444
IP  - 1
DP  - 1999 Feb 5
TI  - Transcription factor AP-2 activity is modulated by protein kinase A-mediated
      phosphorylation.
PG  - 27-31
AB  - We recently reported that APOE promoter activity is stimulated by cAMP, this
      effect being mediated by factor AP-2 [Garcia et al. (1996) J. Neurosci. 16,
      7550-7556]. Here, we study whether cAMP-induced phosphorylation modulates the
      activity of AP-2. Recombinant AP-2 was phosphorylated in vitro by protein kinase 
      A (PKA) at Ser239. Mutation of Ser239 to Ala abolished in vitro phosphorylation
      of AP-2 by PKA, but not the DNA binding activity of AP-2. Cotransfection studies 
      showed that PKA stimulated the effect of AP-2 on the APOE promoter, but not that 
      of the S239A mutant. Therefore, cAMP may modulate AP-2 activity by PKA-induced
      phosphorylation of this factor.
FAU - Garcia, M A
AU  - Garcia MA
AD  - Centro de Biologia Molecular Severo Ochoa, CSIC-Universidad Autonoma de Madrid,
      Spain.
FAU - Campillos, M
AU  - Campillos M
FAU - Marina, A
AU  - Marina A
FAU - Valdivieso, F
AU  - Valdivieso F
FAU - Vazquez, J
AU  - Vazquez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Apolipoproteins E)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - 452VLY9402 (Serine)
RN  - 65189-71-1 (kemptide)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Substitution
MH  - Apolipoproteins E/genetics
MH  - Binding Sites/radiation effects
MH  - Cyclic AMP/physiology
MH  - Cyclic AMP-Dependent Protein Kinases/genetics/*metabolism
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Oligonucleotide Probes/metabolism
MH  - Oligopeptides/pharmacology
MH  - Peptide Fragments/chemical synthesis/pharmacology
MH  - Phosphorylation/drug effects
MH  - Promoter Regions, Genetic/genetics
MH  - Recombinant Fusion Proteins/genetics/isolation & purification/metabolism
MH  - Sequence Deletion
MH  - Serine/genetics/metabolism/physiology
MH  - Transcription Factor AP-2
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Ultraviolet Rays
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0014-5793(99)00021-6 [pii]
AID - 10.1016/s0014-5793(99)00021-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Feb 5;444(1):27-31. doi: 10.1016/s0014-5793(99)00021-6.