PMID- 10036303
OWN - NLM
STAT- MEDLINE
DCOM- 19990407
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 65
IP  - 1
DP  - 1999 Feb 19
TI  - Down regulation of the prepro-orexin gene expression in genetically obese mice.
PG  - 14-22
AB  - The gene expression of prepro-orexin, the precursor of orexin-A and orexin-B
      which are hypothalamic pepetides that are associated with feeding behavior, were 
      examined in control (C57B1/6J) and obese (ob/ob and db/db) mice using in situ
      hybridization histochemistry. Orexins are identical with hypocretins that have
      been identified by directional tag PCR subtractive hybridization method. In situ 
      hybridization histochemistry revealed that the expression of the prepro-orexin
      gene was significantly decreased in ob/ob and db/db mice compared with control
      mice. The gene expression of neuropeptide Y (NPY), a potent feeding stimulant, is
      known to be increased in ob/ob and db/db mice. The expression of the NPY gene in 
      the arcuate nucleus was increased remarkably in ob/ob and db/db mice compared to 
      that of control mice. An immunohistochemical study showed that orexin-A and
      orexin-B immunoreactive neurons exhibited in the lateral and posterior
      hypothalamic areas and the perifornical nucleus were distributed similarly in
      control, ob/ob and db/db mice. These results suggest that the regulatory
      mechanism of orexins/hypocretins may be different from that of NPY in genetically
      obese mice.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Yamamoto, Y
AU  - Yamamoto Y
AD  - Department of Physiology, School of Medicine, University of Occupational and
      Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.
      yama@med.uoeh-u.ac.jp
FAU - Ueta, Y
AU  - Ueta Y
FAU - Date, Y
AU  - Date Y
FAU - Nakazato, M
AU  - Nakazato M
FAU - Hara, Y
AU  - Hara Y
FAU - Serino, R
AU  - Serino R
FAU - Nomura, M
AU  - Nomura M
FAU - Shibuya, I
AU  - Shibuya I
FAU - Matsukura, S
AU  - Matsukura S
FAU - Yamashita, H
AU  - Yamashita H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Blood Glucose)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Probes)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neuropeptides)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Orexins)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Brain Chemistry/genetics
MH  - Carrier Proteins/*genetics
MH  - DNA Probes
MH  - Gene Expression Regulation/physiology
MH  - Hypothalamic Area, Lateral/chemistry/physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese/*physiology
MH  - Neuropeptide Y/genetics
MH  - Neuropeptides/*genetics
MH  - Neurotransmitter Agents/genetics
MH  - Obesity/*genetics
MH  - Orexins
MH  - Protein Precursors/*genetics
MH  - RNA, Messenger/analysis
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0169328X98003209 [pii]
AID - 10.1016/s0169-328x(98)00320-9 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Feb 19;65(1):14-22. doi:
      10.1016/s0169-328x(98)00320-9.