PMID- 10036235
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20051117
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 6)
DP  - 1999 Mar
TI  - ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in
      cortical actin rearrangements.
PG  - 855-66
AB  - The ARF6 GTPase regulates a novel endosomal-plasma membrane recycling pathway and
      influences cortical actin remodeling. Here we examined the relationship between
      ARF6 and Rac1, a Rho family GTPase, implicated in cortical actin rearrangements. 
      Endogenous Rac1 colocalized with ARF6 at the plasma membrane and on the ARF6
      recycling endosome in untransfected HeLa and primary human fibroblast cells. In
      transfected HeLa cells Rac1 and ARF6 also colocalized. Cells expressing wild-type
      ARF6 or Rac1 formed actin-containing surface protrusions and membrane ruffles,
      respectively, upon treatment with the G protein activator aluminum fluoride.
      Aluminum fluoride-treatment of cells transfected with equivalent amounts of
      plasmid resulted in enhanced membrane ruffling, with protrusions appearing as Rac
      expression was lowered. Co-expression of the dominant negative, GTP
      binding-defective ARF6 T27N mutant inhibited the aluminum fluoride-induced
      ruffling observed in cells expressing Rac1, and the constitutive ruffling
      observed in cells expressing the activated Rac1 Q61L mutant. In contrast,
      co-expression of the GTP-binding-defective, T17N mutant of either Rac1 or Cdc42
      with ARF6 did not inhibit the aluminum fluoride-induced surface protrusions, nor 
      did inactivation of Rho with C3-transferase. These observations suggest that
      ARF6, a non-Rho family GTPase, can, by itself, alter cortical actin and can
      influence the ability of Rac1 to form lamellipodia, in part, by regulating its
      trafficking to the plasma membrane.
FAU - Radhakrishna, H
AU  - Radhakrishna H
AD  - Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, MD
      20892, USA.
FAU - Al-Awar, O
AU  - Al-Awar O
FAU - Khachikian, Z
AU  - Khachikian Z
FAU - Donaldson, J G
AU  - Donaldson JG
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - Actins/*metabolism
MH  - Amino Acid Substitution
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Carrier Proteins/*metabolism
MH  - Cell Membrane/*physiology/*ultrastructure
MH  - Cricetinae
MH  - GTP-Binding Proteins/genetics/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Models, Biological
MH  - Mutagenesis, Site-Directed
MH  - Recombinant Proteins/metabolism
MH  - Transfection
MH  - rac GTP-Binding Proteins
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Mar;112 ( Pt 6):855-66.