PMID- 10036191
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Feb 15
TI  - Cloning and characterization of the murine genes for bHLH-ZIP transcription
      factors TFEC and TFEB reveal a common gene organization for all MiT subfamily
      members.
PG  - 111-20
AB  - The microphthalmia-TFE (MiT) subfamily of basic helix-loop-helix leucine zipper
      (bHLH-ZIP) transcription factors, including TFE3, TFEB, TFEC, and Mitf, has been 
      implicated in the regulation of tissue-specific gene expression in several cell
      lineages. In this report, we investigate the genomic organization and structural 
      relatedness of MiT transcription factors. We characterized the gene for mTFEC,
      which covers a region of more than 50 kb and is composed of seven exons. Further,
      we cloned a cDNA for the murine TFEB homologue and characterized its genomic
      structure. The eight coding exons of mTFEB are distributed over a 6-kb region. A 
      multiple alignment of amino acid sequences of known MiT subfamily members
      indicates undescribed, conserved N-terminal regions and common putative
      phosphorylation sites for TFE3, TFEB, and Mitf. Also, intron-exon borders for
      characterized MiT genes appear completely conserved. A new family member and
      closely related putative transcription factor in Caenorhabditis elegans was
      identified by database searches that show a similar genomic organization within
      the bHLH-ZIP region and the acidic domain. Evolutionary aspects and implications 
      for structure-function relationships are discussed.
CI  - Copyright 1999 Academic Press.
FAU - Rehli, M
AU  - Rehli M
AD  - Department of Biochemistry, University of Queensland, Brisbane, Queensland,
      Q4072, Australia.
FAU - Den Elzen, N
AU  - Den Elzen N
FAU - Cassady, A I
AU  - Cassady AI
FAU - Ostrowski, M C
AU  - Ostrowski MC
FAU - Hume, D A
AU  - Hume DA
LA  - eng
SI  - GENBANK/AF077742
SI  - GENBANK/AF079095
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (TFE3 protein, human)
RN  - 0 (TFEC protein, human)
RN  - 0 (Tcfeb protein, mouse)
RN  - 0 (Tcfec protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 136896-33-8 (Tcfe3 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
MH  - Caenorhabditis elegans/genetics
MH  - Conserved Sequence/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Evolution, Molecular
MH  - Helix-Loop-Helix Motifs/*genetics
MH  - Humans
MH  - Leucine Zippers/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0888-7543(98)95588-4 [pii]
AID - 10.1006/geno.1998.5588 [doi]
PST - ppublish
SO  - Genomics. 1999 Feb 15;56(1):111-20. doi: 10.1006/geno.1998.5588.