PMID- 10036189
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20120626
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Feb 15
TI  - The PISSLRE gene: structure, exon skipping, and exclusion as tumor suppressor in 
      breast cancer.
PG  - 90-7
AB  - In sporadic breast cancer, loss of heterozygosity (LOH) frequently occurs in
      three discrete regions of the long arm of chromosome 16q, the most telomeric of
      which is located at 16q24.3. Among the genes mapped to this region, PISSLRE is a 
      plausible candidate tumor suppressor gene. It codes for a putative
      cyclin-dependent kinase that, as with other members of this family, is likely to 
      be involved in regulating the cell cycle and therefore may have a role in
      oncogenesis. We characterized the genomic structure of PISSLRE and found that the
      splicing of this gene is complex. A variety of different transcripts were
      identified, including those due to cryptic splice sites, exon skipping, insertion
      of intronic sequences, and exon scrambling. The last phenomenon was observed in a
      rare PISSLRE transcript in which exons are joined at a nonconsensus splice site
      in an order different from that predicted by the genomic sequence. To screen the 
      PISSLRE gene in breast tumors with ascertained LOH at 16q24.3, we have analyzed
      each exon by single-strand conformational polymorphism. No variation was found in
      the coding sequence, leading us to conclude that another tumor suppressor must be
      targeted by LOH in sporadic breast cancer.
CI  - Copyright 1999 Academic Press.
FAU - Crawford, J
AU  - Crawford J
AD  - Department of Cytogenetics and Molecular Genetics, Adelaide Women's and
      Children's Hospital, North Adelaide, South Australia, 5006, Australia.
FAU - Ianzano, L
AU  - Ianzano L
FAU - Savino, M
AU  - Savino M
FAU - Whitmore, S
AU  - Whitmore S
FAU - Cleton-Jansen, A M
AU  - Cleton-Jansen AM
FAU - Settasatian, C
AU  - Settasatian C
FAU - d'apolito, M
AU  - d'apolito M
FAU - Seshadri, R
AU  - Seshadri R
FAU - Pronk, J C
AU  - Pronk JC
FAU - Auerbach, A D
AU  - Auerbach AD
FAU - Verlander, P C
AU  - Verlander PC
FAU - Mathew, C G
AU  - Mathew CG
FAU - Tipping, A J
AU  - Tipping AJ
FAU - Doggett, N A
AU  - Doggett NA
FAU - Zelante, L
AU  - Zelante L
FAU - Callen, D F
AU  - Callen DF
FAU - Savoia, A
AU  - Savoia A
LA  - eng
SI  - GENBANK/AJ010342
SI  - GENBANK/AJ010343
SI  - GENBANK/AJ010344
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.22 (CDK10 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Alternative Splicing
MH  - Blotting, Northern
MH  - Breast Neoplasms/*genetics
MH  - Chromosomes, Human, Pair 16/genetics
MH  - Cyclin-Dependent Kinases/*genetics
MH  - Exons
MH  - Female
MH  - Gene Amplification
MH  - Genes, Tumor Suppressor/genetics
MH  - Humans
MH  - Loss of Heterozygosity/genetics
MH  - Molecular Sequence Data
MH  - Protein Kinases/*genetics
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0888-7543(98)95676-2 [pii]
AID - 10.1006/geno.1998.5676 [doi]
PST - ppublish
SO  - Genomics. 1999 Feb 15;56(1):90-7. doi: 10.1006/geno.1998.5676.