PMID- 10036188
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20131121
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Feb 15
TI  - M band proteins myomesin and skelemin are encoded by the same gene: analysis of
      its organization and expression.
PG  - 78-89
AB  - The complete exon-intron organization of the murine gene encoding sarcomeric
      myomesin has been determined. The gene is composed of 38 exons and 37 introns,
      spanning approximately 105 kb of DNA. Intron positions and phases are essentially
      identical to those identified in M-protein. They are related to the modular
      structure of myomesin, which is composed almost entirely of immunoglobulin and
      fibronectin type III domains. Nearly all repeats follow a two exon-one domain
      structure. The start and end of each domain are defined by introns in phase I,
      while internal introns are more divergent in position and very rarely use phase
      I. Genomic Southern blotting and reverse transcription-polymerase chain reaction 
      revealed that differential splicing of a single exon gives rise to two
      polypeptides, described in the literature as myomesin and skelemin, respectively.
      A single transcriptional start point was detected in both skeletal and cardiac
      muscle. Analysis of the presumptive promoter region revealed several potential
      regulatory elements. CAT expression assays using promoter deletion constructs
      identified three regions that seem to be important for the muscle-specific
      transcriptional activation of the myomesin gene. These results provide the basis 
      for a comparative analysis of the regulation of myomesin and M-protein genes in
      vivo.
CI  - Copyright 1999 Academic Press.
FAU - Steiner, F
AU  - Steiner F
AD  - Department of Cell Biology, University of Potsdam, Lennestrasse 7a, Potsdam,
      D-14471, Germany.
FAU - Weber, K
AU  - Weber K
FAU - Furst, D O
AU  - Furst DO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Connectin)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (MYOM1 protein, human)
RN  - 0 (Muscle Proteins)
RN  - 0 (Myom1 protein, mouse)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cattle
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Connectin
MH  - Cytoskeletal Proteins/*genetics
MH  - Dogs
MH  - Exons
MH  - Gene Expression
MH  - Haplorhini
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle Proteins/*genetics
MH  - Mutagenesis, Site-Directed
MH  - Promoter Regions, Genetic
MH  - Protein Isoforms/genetics
MH  - Rabbits
MH  - Rats
MH  - Tissue Distribution
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0888-7543(98)95682-8 [pii]
AID - 10.1006/geno.1998.5682 [doi]
PST - ppublish
SO  - Genomics. 1999 Feb 15;56(1):78-89. doi: 10.1006/geno.1998.5682.